Evidence map›Paper›PMID 28102081›Full record

ArticleJournal of proteome research2017

Deep Coverage of Global Protein Expression and Phosphorylation in Breast Tumor Cell Lines Using TMT 10-plex Isobaric Labeling.

Fang-Ke Huang, Guoan Zhang, Kevin Lawlor, Arpi Nazarian, John Philip, Paul Tempst, Noah Dephoure, Thomas A Neubert

Open access · greenAbstract read
In one paragraph

Article in Journal of proteome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 55 citations in OpenAlex.

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  6. Cocaine perturbs mitovesicle biology in the brain.Journal of extracellular vesicles · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Fang-Ke HuangKimmel Center for Biology and Medicine at the Skirball Institute, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine , New York, New York 10016, United States.
Guoan ZhangKimmel Center for Biology and Medicine at the Skirball Institute, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine , New York, New York 10016, United States.
Kevin LawlorMolecular Biology Program, Memorial Sloan Kettering Cancer Center , New York, New York 10065, United States.
Arpi NazarianMolecular Biology Program, Memorial Sloan Kettering Cancer Center , New York, New York 10065, United States.
John PhilipMolecular Biology Program, Memorial Sloan Kettering Cancer Center , New York, New York 10065, United States.
Paul TempstMolecular Biology Program, Memorial Sloan Kettering Cancer Center , New York, New York 10065, United States.
Noah DephoureSandra and Edward Meyer Cancer Center, Department of Biochemistry, Weill Cornell Medical College , New York, New York 10065, United States.
Thomas A NeubertKimmel Center for Biology and Medicine at the Skirball Institute, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine , New York, New York 10016, United States.ORCID 0000-0001-7049-2088
Memorial Sloan Kettering Cancer Center · USNew York University · USCornell University · US

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4M
LTQ-Orbitrap Mass Spectrometer.S10RR027990 · NCRR · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI NEUBERT, THOMAS A · 2011 to 2011
$600k
Integrated, Modular Equipment for Large-scale and Small-scale Peptide SynthesisS10RR023796 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WARING, ALAN J · 2008 to 2008
$224k
NCI NIH HHS P30 CA008748NCRR NIH HHS S10 RR023796NCRR NIH HHS S10 RR027990
6 · The paper itself

Abstract

Labeling peptides with isobaric tags is a popular strategy in quantitative bottom-up proteomics. In this study, we labeled six breast tumor cell lysates (1.34 mg proteins per channel) using 10-plex tandem mass tag reagents and analyzed the samples on a Q Exactive HF Quadrupole-Orbitrap mass spectrometer. We identified a total of 8,706 proteins and 28,186 phosphopeptides, including 7,394 proteins and 23,739 phosphosites common to all channels. The majority of technical replicates correlated with a R

Indexed as

Staining and LabelingBreast NeoplasmsCell Line, TumorCluster AnalysisErb-b2 Receptor Tyrosine KinasesFemaleHumansMass SpectrometryPhosphopeptidesPhosphorylationProteomicsTyrosineERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesPhosphopeptidesTyrosinehierarchical clusteringmass spectrometryphosphoproteomicsquantitative proteomicstandem mass tagtitanium dioxidetyrosine phosphorylation

Identifiers

PMID28102081
PMCPMC5336479
OpenAlexW2574271406

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.