ArticleJournal of computational chemistry2017
Extreme biophysics: Enzymes under pressure.
Article in Journal of computational chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Structural modifications and kinetic effects ofFrontiers in molecular biosciences · 2024Article
- Pressure Adaptations in Deep-SeaBiology · 2021Article
- Adaptations for Pressure and Temperature in Dihydrofolate Reductases.Microorganisms · 2021Article
- Article
- How adding a single methylene to dihydrofolate reductase can change its conformational dynamics.The Journal of chemical physics · 2021Article
- Site-Specific Tryptophan Labels Reveal Local Microsecond-Millisecond Motions of Dihydrofolate Reductase.Molecules (Basel, Switzerland) · 2020Article
- Properties and Applications of Extremozymes from Deep-Sea Extremophilic Microorganisms: A Mini Review.Marine drugs · 2019Review
- In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure.Scientific reports · 2019Article
- Effects of Pressure and Temperature on the Atomic Fluctuations of Dihydrofolate Reductase from a Psychropiezophile and a Mesophile.International journal of molecular sciences · 2019Article
- Cryo-Cooling Effect on DHFR Crystal Studied by Replica-Exchange Molecular Dynamics Simulations.Biophysical journal · 2019Article
- Adaptations for Pressure and Temperature Effects on Loop Motion inHigh pressure research · 2019Article
- Virial Based Berendsen Barostat on GPUs using AMOEBA in Tinker-OpenMM.Results in chemistry · 2019Article
- Enzymes from piezophiles.Seminars in cell & developmental biology · 2018Review
- High-pressure protein crystal structure analysis of Escherichia coli dihydrofolate reductase complexed with folate and NADPActa crystallographica. Section D, Structural biology · 2018Article
- Quasiharmonic Analysis of the Energy Landscapes of Dihydrofolate Reductase from Piezophiles and Mesophiles.The journal of physical chemistry. B · 2018Article
- Quasiharmonic analysis of protein energy landscapes from pressure-temperature molecular dynamics simulations.The Journal of chemical physics · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A critical question about piezophilic (pressure-loving) microbes is how their constituent molecules maintain function under high pressure. Here, factors are examined that may lead to the increased activity under pressure in dihydrofolate reductase from the piezophilic Moritella profunda compared to the homologous enzyme from the mesophilic Escherichia coli. Molecular dynamics simulations are performed at various temperatures and pressures to examine how pressure affects the flexibility of the enzymes from these two microbes, since both stability and flexibility are necessary for enzyme activity. The results suggest that collective motions on the 10-ns timescale are responsible for the flexibility necessary for "corresponding states" activity at the growth conditions of the parent organism. In addition, the results suggest that while the lower stability of many enzymes from deep-sea microbes may be an adaptation for greater flexibility at low temperatures, high pressure may enhance their adaptation to low temperatures. © 2017 Wiley Periodicals, Inc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.