Evidence map›Paper›PMID 28101808›Full record

ArticleJournal of industrial microbiology & biotechnology2017

Construction of engineered Saccharomyces cerevisiae strain to improve that whole-cell biocatalytic production of melibiose from raffinose.

Yingbiao Zhou, Yueming Zhu, Yan Men, Caixia Dong, Yuanxia Sun, Juankun Zhang

Abstract read
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In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Biosynthesis of Raffinose and Stachyose from Sucrose via anApplied and environmental microbiology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Yingbiao ZhouKey Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Key Laboratory of Industrial Microbiology of Tianjin, College of Bioengineering, Tianjin University of Science and Technology, No. 32, 13th. Avenue, Tianjin Economic and Development Area, Tianjin, 300457, China.
Yueming ZhuNational Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China.
Yan MenNational Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China.
Caixia DongTianjin Key Laboratory on Technology Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
Yuanxia SunNational Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China. syx0430@hotmail.com.
Juankun ZhangKey Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Key Laboratory of Industrial Microbiology of Tianjin, College of Bioengineering, Tianjin University of Science and Technology, No. 32, 13th. Avenue, Tianjin Economic and Development Area, Tianjin, 300457, China. Zkz_12@126.com.
Chinese Academy of Sciences · CNTianjin University of Science and Technology · CNTianjin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There are excessive by-products in the biocatalysis process of this whole-cell biocatalytic production of melibiose from raffinose with current Saccharomyces cerevisiae strains. To solve this problem, we constructed engineered strains based on a liquor yeast (S. cerevisiae) via gene deletion (mel1 gene), heterologous integration (fsy1 or/and ffzi1 gene from Candida magnoliae), and gene overexpression (gcr1 gene). Functional verification showed that deletion of the mel1 gene led to elimination of the reactions catalyzed by α-galactosidase, as well as elimination of the degradation of melibiose and the formation of galactose by-product. Insertion of the fsy1 or/and ffzi1 gene and overexpression of the gcr1 gene could contribute to fructose transport for enhancing the biopurification rate of the fructose by-product. Compared with the wild-type strain, the optimal engineered strain of MP8 (Δmel1::fsy1

Indexed as

alpha-GalactosidaseBiocatalysisFructoseGalactoseGene DeletionIndustrial MicrobiologyMelibioseMicroorganisms, Genetically-ModifiedProtein EngineeringRaffinoseSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteinsalpha-GalactosidaseFructoseGalactoseMelibioseRaffinoseSaccharomyces cerevisiae ProteinsBiopurificationEngineered strainMelibioseRaffinoseSaccharomyces cerevisiaeWhole-cell biocatalysis

Identifiers

PMID28101808
OpenAlexW2574378222

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.