ArticleBiotechnology for biofuels2017
Exploring accessibility of pretreated poplar cell walls by measuring dynamics of fluorescent probes.
Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 39 citations in OpenAlex.
- Wheat straw: A natural remedy against different maladies.Food science & nutrition · 2021Review
- Aqueous Ammonia Pre-treatment of Wheat Straw: Process Optimization and Broad Spectrum Dye Adsorption on Nitrogen-Containing Lignin.Frontiers in chemistry · 2019Article
- Distribution, mobility, and anchoring of lignin-related oxidative enzymes in Arabidopsis secondary cell walls.Journal of experimental botany · 2018Article
- Fluorescent Nano-Probes to Image Plant Cell Walls by Super-Resolution STED Microscopy.Plants (Basel, Switzerland) · 2018Article
- Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.Biotechnology for biofuels · 2018Article
- FRET-SLiM on native autofluorescence: a fast and reliable method to study interactions between fluorescent probes and lignin in plant cell wall.Plant methods · 2018Article
- Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.Biotechnology for biofuels · 2018Article
- Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type.Scientific reports · 2017Article
- Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method.Biotechnology for biofuels · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe lignocellulosic cell wall network is resistant to enzymatic degradation due to the complex chemical and structural features. Pretreatments are thus commonly used to overcome natural recalcitrance of lignocellulose. Characterization of their impact on architecture requires combinatory approaches. However, the accessibility of the lignocellulosic cell walls still needs further insights to provide relevant information.
resultsPoplar specimens were pretreated using different conditions. Chemical, spectral, microscopic and immunolabeling analysis revealed that poplar cell walls were more altered by sodium chlorite-acetic acid and hydrothermal pretreatments but weakly modified by soaking in aqueous ammonium. In order to evaluate the accessibility of the pretreated poplar samples, two fluorescent probes (rhodamine B-isothiocyanate-dextrans of 20 and 70 kDa) were selected, and their mobility was measured by using the fluorescence recovery after photobleaching (FRAP) technique in a full factorial experiment. The mobility of the probes was dependent on the pretreatment type, the cell wall localization (secondary cell wall and cell corner middle lamella) and the probe size. Overall, combinatory analysis of pretreated poplar samples showed that even the partial removal of hemicellulose contributed to facilitate the accessibility to the fluorescent probes. On the contrary, nearly complete removal of lignin was detrimental to accessibility due to the possible cellulose-hemicellulose collapse.
conclusionsEvaluation of plant cell wall accessibility through FRAP measurement brings further insights into the impact of physicochemical pretreatments on lignocellulosic samples in combination with chemical and histochemical analysis. This technique thus represents a relevant approach to better understand the effect of pretreatments on lignocellulose architecture, while considering different limitations as non-specific interactions and enzyme efficiency.
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