ArticleJournal of biological engineering2017
Single cell isolation process with laser induced forward transfer.
Article in Journal of biological engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 37 citations in OpenAlex.
- Unlocking Microbial Dark Matter: A Comprehensive Review of Isolation Technologies from Traditional Culturing to Single-Cell Technologies.Microorganisms · 2026Review
- Microbial single-cell omics in situ.Cell genomics · 2026Article
- High-throughput single-cell sorting by stimulated Raman-activated cell ejection.Science advances · 2024Article
- A Microfluidic Chip for Single-Cell Capture Based on Stagnation Point Flow and Boundary Effects.Micromachines · 2024Article
- LIFTOSCOPE: development of an automated AI-based module for time-effective and contactless analysis and isolation of cells in microtiter plates.Journal of biological engineering · 2023Article
- Laser transfer for circulating tumor cell isolation in liquid biopsy.International journal of bioprinting · 2023Article
- Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink.Micromachines · 2021Article
- 3D bioprinting and its potential impact on cardiac failure treatment: An industry perspective.APL bioengineering · 2020Article
- New microorganism isolation techniques with emphasis on laser printing.International journal of bioprinting · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA viable single cell is crucial for studies of single cell biology. In this paper, laser-induced forward transfer (LIFT) was used to isolate individual cell with a closed chamber designed to avoid contamination and maintain humidity. Hela cells were used to study the impact of laser pulse energy, laser spot size, sacrificed layer thickness and working distance. The size distribution, number and proliferation ratio of separated cells were statistically evaluated. Glycerol was used to increase the viscosity of the medium and alginate were introduced to soften the landing process.
resultsThe role of laser pulse energy, the spot size and the thickness of titanium in energy absorption in LIFT process was theoretically analyzed with Lambert-Beer and a thermal conductive model. After comprehensive analysis, mechanical damage was found to be the dominant factor affecting the size and proliferation ratio of the isolated cells. An orthogonal experiment was conducted, and the optimal conditions were determined as: laser pulse energy, 9 μJ; spot size, 60 μm; thickness of titanium, 12 nm; working distance, 700 μm;, glycerol, 2% and alginate depth, greater than 1 μm. With these conditions, along with continuous incubation, a single cell could be transferred by the LIFT with one shot, with limited effect on cell size and viability.
conclusionLIFT conducted in a closed chamber under optimized condition is a promising method for reliably isolating single cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.