ArticleFrontiers in plant science2016
Laser Capture Microdissection Protocol for Xylem Tissues of Woody Plants.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 41 citations in OpenAlex.
- Serial Spatial Transcriptomes Reveal Regulatory Transitions in Maize Leaf Development.Plant biotechnology journal · 2026Article
- A pennycress transparent testa 8 knockout mutant has drastic changes in seed coat anatomy and chemical compositions.Journal of experimental botany · 2026Article
- Co-extraction of Small RNAs and Total RNA from Arbuscular Mycorrhizal Roots Using Laser Microdissection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq.Plant methods · 2025Article
- How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis.Plant methods · 2023Review
- Organellar microcapture to extract nuclear and plastid DNA from recalcitrant wood specimens and trace evidence.Plant methods · 2022Article
- Xylem Parenchyma-Role and Relevance in Wood Functioning in Trees.Plants (Basel, Switzerland) · 2021Review
- Tissue-specific transcriptome profiling of the Arabidopsis inflorescence stem reveals local cellular signatures.The Plant cell · 2021Article
- Laser Microdissection of Specific Stem-Base Tissue Types from Olive Microcuttings for Isolation of High-Quality RNA.Biology · 2021Article
- An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root.STAR protocols · 2020Article
- Article
- Ray Parenchymal Cells Contribute to Lignification of Tracheids in Developing Xylem of Norway Spruce.Plant physiology · 2019Article
- A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis.Plant methods · 2019Article
- An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK!Methods in molecular biology (Clifton, N.J.) · 2019Article
- Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays.Nature protocols · 2018Article
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Authors and funding
7 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laser capture microdissection (LCM) enables precise dissection and collection of individual cell types from complex tissues. When applied to plant cells, and especially to woody tissues, LCM requires extensive optimization to overcome such factors as rigid cell walls, large central vacuoles, intercellular spaces, and technical issues with thickness and flatness of the sections. Here we present an optimized protocol for the laser-assisted microdissection of developing xylem from mature trees: a gymnosperm (Norway spruce,
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