ArticleOpen biology2017
Zika virus causes supernumerary foci with centriolar proteins and impaired spindle positioning.
Article in Open biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- HMC3 revealed: how much do these "Microglia" really tell us?Frontiers in immunology · 2026Review
- Defective Neural Stem and Progenitor Cell Proliferation in Neurodevelopmental Disorders.Journal of developmental biology · 2025Review
- Emerging Arthropod-Borne Viruses Hijack the Host Cell Cytoskeleton During Neuroinvasion.Viruses · 2025Review
- Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells.Microorganisms · 2023Article
- Zika virus alters centrosome organization to suppress the innate immune response.EMBO reports · 2022Article
- Zika Virus Induces Mitotic Catastrophe in Human Neural Progenitors by Triggering Unscheduled Mitotic Entry in the Presence of DNA Damage While Functionally Depleting Nuclear PNKP.Journal of virology · 2022Article
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- Article
- Zika Virus Overview: Transmission, Origin, Pathogenesis, Animal Model and Diagnosis.Zoonoses (Shannon, Ireland) · 2021Article
- Neurocognitive impacts of arbovirus infections.Journal of neuroinflammation · 2020Review
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- Human Brain Organoids to Decode Mechanisms of Microcephaly.Frontiers in cellular neuroscience · 2020Review
- Zika virus increases mind bomb 1 levels, causing degradation of pericentriolar material 1 (PCM1) and dispersion of PCM1-containing granules from the centrosome.The Journal of biological chemistry · 2019Article
- HCV and flaviviruses hijack cellular mechanisms for nuclear STAT2 degradation: Up-regulation of PDLIM2 suppresses the innate immune response.PLoS pathogens · 2019Article
- Pathophysiology and Mechanisms of Zika Virus Infection in the Nervous System.Annual review of neuroscience · 2019Review
- Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells.Nature communications · 2019Article
- Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.Neuron · 2019Article
- Viral Infection or IFN-α Alters Mitotic Spindle Orientation by Modulating Pericentrin Levels.iScience · 2019Article
- The Role of Host Cytoskeleton in Flavivirus Infection.Virologica Sinica · 2019Review
- Acute Lengthening of Progenitor Mitosis Influences Progeny Fate during Cortical Development in vivo.Developmental neuroscience · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zika virus (ZIKV) causes congenital microcephaly. Although ZIKV can impair cell cycle progression and provoke apoptosis, which probably contributes to disease aetiology through depletion of neural progenitor cells, additional cellular mechanisms may be important. Here, we investigated whether ZIKV infection alters centrosome number and spindle positioning, because such defects are thought to be at the root of inherited primary autosomal recessive microcephaly (MCPH). In addition to HeLa cells, in which centrosome number and spindle positioning can be well monitored, we analysed retinal epithelial cells (RPE-1), as well as brain-derived microglial (CHME-5) and neural progenitor (ReN) cells, using immunofluorescence. We established that ZIKV infection leads to supernumerary foci containing centriolar proteins that in some cases drive multipolar spindle assembly, as well as spindle positioning defects in HeLa, RPE-1 and CHME-5 cells, but not in ReN cells. We uncovered similar phenotypes in HeLa cells upon infection with dengue virus (DENV-2), another flavivirus that does not target brain cells and does not cause microcephaly. We conclude that infection with Flaviviridae can increase centrosome numbers and impair spindle positioning, thus potentially contributing to microcephaly in the case of Zika.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.