ArticleMicrobiology (Reading, England)2017
Environment-directed activation of the Escherichia coliflhDC operon by transposons.
Article in Microbiology (Reading, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- Investigating the Trans Effects of IS<italic>1</italic> Transposases on Intragenomic DNA Movements in <italic>Escherichia coli</italic>.Microbial physiology · 2026Article
- Examining the Roles of Genomic Context and Endogenous Regulatory Elements on ISInternational journal of molecular sciences · 2025Article
- Distantly related bacteria share a rigid proteome allocation strategy with flexible enzyme kinetics.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Role of a single MCP in evolutionary adaptation ofApplied and environmental microbiology · 2025Article
- Transcriptional mechanism by which IS5 activates the fucAO operon in Escherichia coli.Nucleic acids research · 2025Article
- Investigating How Genomic Contexts Impact IS5 Transposition Within theMicroorganisms · 2024Article
- The Transposition of Insertion Sequences in Sigma-Factor- and LysR-Deficient Mutants ofMicroorganisms · 2024Article
- The effect of DNA-binding proteins on insertion sequence element transposition upstream of theFrontiers in microbiology · 2024Article
- Mechanism ofInternational journal of molecular sciences · 2023Article
- Revisiting Fur Regulon Leads to a Comprehensive Understanding of Iron and Fur Regulation.International journal of molecular sciences · 2023Article
- Laboratory strains ofMicrobial genomics · 2023Article
- Effects of Global and Specific DNA-Binding Proteins on Transcriptional Regulation of theInternational journal of molecular sciences · 2022Article
- Active Transposition of Insertion Sequences in Prokaryotes: Insights from the Response ofAntioxidants (Basel, Switzerland) · 2022Review
- Frequency, composition and mobility of Escherichia coli-derived transposable elements in holdings of plasmid repositories.Microbial biotechnology · 2022Article
- Insertion Sequence (IS) Element-Mediated Activating Mutations of the Cryptic Aromatic β-Glucoside Utilization (International journal of molecular sciences · 2022Article
- Gain of SpontaneousFrontiers in bioengineering and biotechnology · 2021Article
- Rapid Accumulation of Motility-Activating Mutations in Resting Liquid Culture of Escherichia coli.Journal of bacteriology · 2019Article
- Intensive targeting of regulatory competence genes by transposable elements in streptococci.Molecular genetics and genomics : MGG · 2019Article
- A shifting mutational landscape in 6 nutritional states: Stress-induced mutagenesis as a series of distinct stress input-mutation output relationships.PLoS biology · 2017Article
- Hopping into a hot seat: Role of DNA structural features on IS5-mediated gene activation and inactivation under stress.PloS one · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The flagellar system in Escherichia coli K12 is expressed under the control of the flhDC-encoded master regulator FlhDC. Transposition of insertion sequence (IS) elements to the upstream flhDC promoter region up-regulates transcription of this operon, resulting in a more rapid motility. Wang and Wood (ISME J 2011;5:1517-1525) provided evidence that insertion of IS5 into upstream activating sites occurs at higher rates in semi-solid agar media in which swarming behaviour is allowed as compared with liquid or solid media where swarming cannot occur. We confirm this conclusion and show that three IS elements, IS1, IS3 and IS5, transpose to multiple upstream sites within a 370 bp region of the flhDC operon control region. Hot spots for IS insertion correlate with positions of stress-induced DNA duplex destabilization (SIDD). We show that IS insertion occurs at maximal rates in 0.24 % agar, with rates decreasing dramatically with increasing or decreasing agar concentrations. In mixed cultures, we show that these mutations preferentially arise from the wild-type parent at frequencies of up to 3×10-3 cell-1 day-1 when the inoculated parental and co-existing IS-activated mutant cells are entering the stationary growth phase. We rigorously show that the apparent increased mutation frequencies cannot be accounted for by increased swimming or by increased growth under the selective conditions used. Thus, our data are consistent with the possibility that appropriate environmental conditions, namely those that permit but hinder flagellar rotation, result in the activation of a mutational pathway that involves IS element insertion upstream of the flhDC operon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.