ArticleBMC research notes2017
Evaluation of three sample preparation methods for the direct identification of bacteria in positive blood cultures by MALDI-TOF.
Article in BMC research notes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Isolating viable bacteria from positive blood cultures: impedance cytometry analysis.Biomedical microdevices · 2026Article
- Direct identification and susceptibility testing of positive blood cultures: a large-scale evaluation of an ammonium chloride-based in-house workflow.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Rapid Bacterial Identification and Quantitative Antimicrobial Susceptibility Assessment from Positive Blood Cultures to Optimize Bloodstream Infection Management.Microorganisms · 2026Article
- Evaluation of the Application of PCR and MALDI-TOF MS Methods for the Identification ofPathogens (Basel, Switzerland) · 2026Article
- Accuracy of two Sepsityper MALDI-TOF MS methods for bacterial identification in bloodstream infections in dogs, foals, and calves using Bayesian latent class model.The veterinary quarterly · 2025Article
- Direct identification and antimicrobial susceptibility testing of microorganisms from positive blood culture bottles using a membrane filtration method.BMC microbiology · 2025Article
- Bacterial identification using MALDI-TOF mass spectrometry in positive blood cultures: A pilot study.Journal of biological methods · 2025Article
- Evaluation of EUCAST Rapid Antimicrobial Susceptibility Testing for Gram-Negative Bacteria Directly from Positive Blood Cultures.Infection and drug resistance · 2025Article
- Multiplex microarray PCR Unyvero BCU system to accelerate relevant antimicrobial treatment in polymicrobial bloodstream infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Antibiotic-Polyphosphate Nanocomplexes: A Promising System for Effective Biofilm Eradication.International journal of nanomedicine · 2024Article
- Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2022Review
- Direct Identification, Antimicrobial Susceptibility Testing, and Extended-Spectrum β-Lactamase and Carbapenemase Detection in Gram-Negative Bacteria Isolated from Blood Cultures.Infection and drug resistance · 2022Article
- Comparison of novel approaches for expedited pathogen identification and antimicrobial susceptibility testing against routine blood culture diagnostics.Letters in applied microbiology · 2021Article
- Impact of Direct From Blood Culture Identification of Pathogens Paired With Antimicrobial Stewardship Interventions in a Pediatric Hospital.The journal of pediatric pharmacology and therapeutics : JPPT : the official journal of PPAG · 2021Article
- Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS.Annals of clinical microbiology and antimicrobials · 2020Article
- Rapid method for direct identification of positive blood cultures by MALDI-TOF MS.Experimental and therapeutic medicine · 2020Article
- Identification and Morphological Characterization of Biofilms Formed by Strains Causing Infection in Orthopedic Implants.Pathogens (Basel, Switzerland) · 2020Article
- Rapid direct identification of positive paediatric blood cultures by MALDI-TOF MS technology and its clinical impact in the paediatric hospital setting.BMC research notes · 2020Article
- Rapid identification of respiratory bacterial pathogens from bronchoalveolar lavage fluid in cattle by MALDI-TOF MS.Scientific reports · 2019Article
- A new culture-based method for rapid identification of microorganisms in polymicrobial blood cultures by MALDI-TOF MS.BMC microbiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPatient mortality is significantly reduced by rapid identification of bacteria from sterile sites. MALDI-TOF can identify bacteria directly from positive blood cultures and multiple sample preparation methods are available. We evaluated three sample preparation methods and two MALDI-TOF score cut-off values. Positive blood culture bottles with organisms present in Gram stains were prospectively analysed by MALDI-TOF. Three lysis reagents (Saponin, SDS, and SepsiTyper lysis bufer) were applied to each positive culture followed by centrifugation, washing and protein extraction steps. Methods were compared using the McNemar test and 16S rDNA sequencing was used to assess discordant results.
resultsIn 144 monomicrobial cultures, using ≥2.000 as the cut-off value, species level identifications were obtained from 69/144 (48%) samples using Saponin, 86/144 (60%) using SDS, and 91/144 (63%) using SepsiTyper. The difference between SDS and SepsiTyper was not statistically significant (P = 0.228). Differences between Saponin and the other two reagents were significant (P < 0.01). Using ≥1.700 plus top three results matching as the cut-off value, species level identifications were obtained from 100/144 (69%) samples using Saponin, 103/144 (72%) using SDS, and 106/144 (74%) using SepsiTyper and there was no statistical difference between the methods. No true discordances between culture and direct MALDI-TOF identification were observed in monomicrobial cultures. In 32 polymicrobial cultures, MALDI-TOF identified one organism in 34-75% of samples depending on the method.
conclusionsThis study demonstrates two inexpensive in-house detergent lysis methods are non-inferior to a commercial kit for analysis of positive blood cultures by direct MALDI-TOF in a clinical diagnostic microbiology laboratory.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.