ArticleBMC genomics2017
Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosine.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Effects of improved on-farm crop storage on DNA methylation of mothers and their infants: evidence from a randomized controlled trial in Kenya.Clinical epigenetics · 2024Trial
- Urinary DNA Methylation and Hydroxymethylation Dynamics as Candidate Biomarkers of Occupational Chemical Exposure-An Exploratory Pilot Study.International journal of molecular sciences · 2026Article
- DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.Genome biology · 2026Article
- Longitudinal Analysis of Estrogen Receptor Gene Methylation, Estradiol, and Depressive Symptoms During the Perinatal Period.Molecular neurobiology · 2025Article
- The interferon γ pathway enhances pluripotency and X-chromosome reactivation in iPSC reprogramming.Science advances · 2024Article
- Personality vulnerability to depression, resilience, and depressive symptoms: epigenetic markers among perinatal women.Upsala journal of medical sciences · 2024Article
- Evaluating Methylation of the Oxytocin Receptor Gene and the Oxytocin Intergenic Region.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Integrative DNA Methylation and Gene Expression Analysis in the Prefrontal Cortex of Mexicans Who Died by Suicide.The international journal of neuropsychopharmacology · 2021Article
- Methylation of the tyrosine hydroxylase gene is dysregulated by cocaine dependence in the human striatum.iScience · 2021Article
- Cocaine-related DNA methylation in caudate neurons alters 3D chromatin structure of the IRXA gene cluster.Molecular psychiatry · 2021Article
- The study protocol: Neuroendocrinology and (epi-) genetics of female reproductive transition phase mood disorder - an observational, longitudinal study from pregnancy to postpartum.BMC pregnancy and childbirth · 2020Article
- Experimental approaches to tracking mobile genetic elements in microbial communities.FEMS microbiology reviews · 2020Review
- DifferentialInternational journal of molecular sciences · 2020Article
- Sleep and Methylation of Estrogen Receptor Genes,Nature and science of sleep · 2020Article
- Integrated genome-wide methylation and expression analyses reveal functional predictors of response to antidepressants.Translational psychiatry · 2019Article
- Epigenetic markers in inflammation-related genes associated with mood disorder: a cross-sectional and longitudinal study in high-risk offspring of bipolar parents.International journal of bipolar disorders · 2019Article
- DNA Methylation in Healthy Older Adults With a History of Childhood Adversity-Findings From the Women 40+ Healthy Aging Study.Frontiers in psychiatry · 2019Article
- Review
- HAM-TBS: high-accuracy methylation measurements via targeted bisulfite sequencing.Epigenetics & chromatin · 2018Article
- Analysis of DNA modifications in aging research.GeroScience · 2018Review
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
backgroundEpigenetic modifications of DNA, such as 5-methylcytosine and 5-hydroxymethycytosine, play important roles in development and disease. Here, we present a cost-effective and versatile methodology for the analysis of DNA methylation in targeted genomic regions, which comprises multiplexed, PCR-based preparation of bisulfite DNA libraries followed by customized MiSeq sequencing.
resultsUsing bisulfite and oxidative bisulfite conversion of DNA, we have performed multiplexed targeted sequencing to analyse several kilobases of genomic DNA in up to 478 samples, and achieved high coverage data of 5-methylcytosine and 5-hydroxymethycytosine at single-base resolution. Our results demonstrate the ability of this methodology to detect all levels of cytosine modifications at greater than 100× coverage in large sample sets at low cost compared to other targeted methods.
conclusionsThis approach can be applied to multiple settings, from candidate gene to clinical studies, and is especially useful for validation of differentially methylated or hydroxymethylated regions following whole-genome analyses.
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