ArticleBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica2017
A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry.
Article in Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 24 citations in OpenAlex.
- Regulation of central carbon metabolism and storage polymers by PTSApplied and environmental microbiology · 2026Article
- State-of-the-art methods for quantifying microbial polyhydroxyalkanoates.Applied and environmental microbiology · 2025Review
- Article
- Community composition and physiological plasticity control microbial carbon storage across natural and experimental soil fertility gradients.The ISME journal · 2023Article
- Multiple CheY Proteins Control Surface-Associated Lifestyles ofFrontiers in microbiology · 2021Article
- Transcriptional Responses ofFrontiers in microbiology · 2021Article
- The use of LipidGreen2 for visualization and quantification of intracellular Poly(3-hydroxybutyrate) inBiochemistry and biophysics reports · 2020Article
- 3-Hydroxybutyrate Derived from Poly-3-Hydroxybutyrate Mobilization Alleviates Protein Aggregation in Heat-Stressed Herbaspirillum seropedicae SmR1.Applied and environmental microbiology · 2020Article
- Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry.Bio-protocol · 2017Article
- Prospecting for Marine Bacteria for Polyhydroxyalkanoate Production on Low-Cost Substrates.Bioengineering (Basel, Switzerland) · 2017Article
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8 authors at 1 institution in 1 country.
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Abstract
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method was successfully applied to the quantification of PHB in epiphytic bacteria isolated from rice roots.
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