ArticlePloS one2017
A New Statistical Approach to Characterize Chemical-Elicited Behavioral Effects in High-Throughput Studies Using Zebrafish.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Zebrafish behavioral assessment as a first-tier whole-organism NAM for developmental neurotoxicity: A multi-laboratory evaluation.Neurotoxicology · 2026Article
- Rapid behavioral screening in the planarianFrontiers in toxicology · 2026Article
- A data analysis approach for locomotor behavioral responses in developmentally exposed zebrafish.NAM journal · 2025Article
- Aryl hydrocarbon receptor-dependent toxicity by retene requires metabolic competence.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Deep autoencoder-based behavioral pattern recognition outperforms standard statistical methods in high-dimensional zebrafish studies.PLoS computational biology · 2024Article
- The zebrafish gut microbiome influences benzo[a]pyrene developmental neurobehavioral toxicity.Scientific reports · 2024Article
- Article
- Diverse PFAS produce unique transcriptomic changes linked to developmental toxicity in zebrafish.Frontiers in toxicology · 2024Article
- Advances in PAH mixture toxicology enabled by zebrafish.Current opinion in toxicology · 2023Article
- Exploring the Influence of Experimental Design on Toxicity Outcomes in Zebrafish Embryo Tests.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Article
- Grouping of chemicals into mode of action classes by automated effect pattern analysis using the zebrafish embryo toxicity test.Archives of toxicology · 2022Article
- Size- and Oxidation-Dependent Toxicity of Graphene Oxide Nanomaterials in Embryonic Zebrafish.Nanomaterials (Basel, Switzerland) · 2022Article
- A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals.International journal of environmental research and public health · 2022Article
- Sulfonamide functional head on short-chain perfluorinated substance drives developmental toxicity.iScience · 2022Article
- The zebrafish embryo as an in vivo model for screening nanoparticle-formulated lipophilic anti-tuberculosis compounds.Disease models & mechanisms · 2022Article
- Behavior Effects of Structurally Diverse Per- and Polyfluoroalkyl Substances in Zebrafish.Chemical research in toxicology · 2021Article
- Developmental toxicity in zebrafish (Danio rerio) exposed to uranium: A comparison with lead, cadmium, and iron.Environmental pollution (Barking, Essex : 1987) · 2021Article
- Systematic Assessment of Exposure Variations on Observed Bioactivity in Zebrafish Chemical Screening.Toxics · 2020Article
- The multi-dimensional embryonic zebrafish platform predicts flame retardant bioactivity.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Zebrafish have become an important alternative model for characterizing chemical bioactivity, partly due to the efficiency at which systematic, high-dimensional data can be generated. However, these new data present analytical challenges associated with scale and diversity. We developed a novel, robust statistical approach to characterize chemical-elicited effects in behavioral data from high-throughput screening (HTS) of all 1,060 Toxicity Forecaster (ToxCast™) chemicals across 5 concentrations at 120 hours post-fertilization (hpf). Taking advantage of the immense scale of data for a global view, we show that this new approach reduces bias introduced by extreme values yet allows for diverse response patterns that confound the application of traditional statistics. We have also shown that, as a summary measure of response for local tests of chemical-associated behavioral effects, it achieves a significant reduction in coefficient of variation compared to many traditional statistical modeling methods. This effective increase in signal-to-noise ratio augments statistical power and is observed across experimental periods (light/dark conditions) that display varied distributional response patterns. Finally, we integrated results with data from concomitant developmental endpoint measurements to show that appropriate statistical handling of HTS behavioral data can add important biological context that informs mechanistic hypotheses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.