ArticleJCI insight2017
Quantitative measurement of lymphatic function in mice by noninvasive near-infrared imaging of a peripheral vein.
Article in JCI insight, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Quantification of Lacteal Contractility by Live Intravital Imaging.Microcirculation (New York, N.Y. : 1994) · 2026Article
- Serum indocyanine green quantification enables objective assessment of lymphatic function.Scientific reports · 2026Article
- Chromenylium and Flavylium Polymethine Fluorophores Light Up the Shortwave Infrared Region.Accounts of chemical research · 2026Article
- Key refinements to a large animal model for measurement of real-time lymphatic transport.BMC medical imaging · 2025Article
- Pannexins in the vasculature.American journal of physiology. Heart and circulatory physiology · 2025Review
- In vivo antibody labeling route and fluorophore dictate labeling efficiency, sensitivity, and longevity.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Chromenylium Star Polymers: Merging Water Solubility and Stealth Properties with Shortwave Infrared Emissive Fluorophores.ACS central science · 2025Article
- A multiresolution approach with method-informed statistical analysis for quantifying lymphatic pumping dynamics.Npj imaging · 2025Article
- Immuno-oncological interactions between meningeal lymphatics and glioblastoma: from mechanisms to therapies.Theranostics · 2025Review
- Impairment of spinal CSF flow precedes immune cell infiltration in an active EAE model.Journal of neuroinflammation · 2024Article
- Longitudinal Intravascular Antibody Labeling Identified Regulatory T Cell Recruitment as a Therapeutic Target in a Mouse Model of Lung Cancer.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Targeting lymphatic function in cardiovascular-kidney-metabolic syndrome: preclinical methods to analyze lymphatic function and therapeutic opportunities.Frontiers in cardiovascular medicine · 2024Review
- Multi-omics analysis identifies IgG2b class-switching with ALCAM-CD6 co-stimulation in joint-draining lymph nodes during advanced inflammatory-erosive arthritis.Frontiers in immunology · 2023Article
- Effects of increased intracranial pressure on cerebrospinal fluid influx, cerebral vascular hemodynamic indexes, and cerebrospinal fluid lymphatic efflux.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022Article
- Central nervous system zoning: How brain barriers establish subdivisions for CNS immune privilege and immune surveillance.Journal of internal medicine · 2022Article
- Magnetic resonance imaging of cerebrospinal fluid outflow after low-rate lateral ventricle infusion in mice.JCI insight · 2022Article
- Modulation of lymphatic transport in the central nervous system.Theranostics · 2022Review
- Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism.Nature cancer · 2021Article
- Multichromatic near-infrared imaging to assess interstitial lymphatic and venous uptake in vivo.Journal of biomedical optics · 2021Article
- Development and Clinical Validation of the LymphMonitor Technology to Quantitatively Assess Lymphatic Function.Diagnostics (Basel, Switzerland) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical imaging methods have been developed to measure lymphatic function in skin; however, the lymphatic system of many organs is not accessible to this technology. Since lymphatic transport of macromolecules from any organ proceeds to the blood circulation, we aimed to develop a method that can measure lymphatic function by monitoring the fluorescence in a superficial vein of an interstitially injected tracer. We selected a 40-kDa PEGylated near-infrared dye conjugate, as it showed lymphatic system-specific uptake and extended circulation in blood. Lymphatic transport to blood from subcutaneous tissue required a transit time before signal enhancement was seen in blood followed by a steady rise in signal over time. Increased lymphatic transport was apparent in awake mice compared with those under continuous anesthesia. The methods were validated in K14-VEGFR-3-Fc and K14-VEGF-C transgenic mice with loss and gain of lymphatic function, respectively. Reduced lymphatic transport to blood was also found in aged mice. The technique was also able to measure lymphatic transport from the peritoneal cavity, a location not suitable for optical imaging. The method is a promising, simple approach for assessment of lymphatic function and for monitoring of therapeutic regimens in mouse models of disease and may have potential for clinical translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.