ArticlePeerJ2017
Handling stress may confound murine gut microbiota studies.
Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Evaluating the Impact of Prophylactic Administration of Ivermectin on the Fecal Microbiome of Healthy C57BL/6J Mice (Mus musculus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Causal Associations Between Gut Microbes and Heart Failure Across Multiple Etiologies: A Mendelian Randomization Study.Reviews in cardiovascular medicine · 2026Article
- BiombalanceAntioxidants (Basel, Switzerland) · 2025Article
- Biogeographical distribution of gut microbiome composition and function is partially recapitulated by fecal transplantation into germ-free mice.The ISME journal · 2025Article
- Colonization byFrontiers in immunology · 2025Article
- Social network dynamics, infant loss, and gut microbiota composition in female Colobus vellerosus during time periods with alpha male challenges.Primates; journal of primatology · 2024Article
- Effect of dietary supplementation with Lactobacillus helveticus R0052 on seizure thresholds and antiseizure potency of sodium valproate in mice.Psychopharmacology · 2024Article
- Possibilities and limits for using the gut microbiome to improve captive animal health.Animal microbiome · 2021Review
- Development of a 3D-Printed High Temperature Resin Cecal Fistula Implant for Long-Term and Minimally Invasive Access to the Gut Microbiome.Nutrients · 2021Article
- Updating salamander datasets with phenotypic and stomach content information for two mainland Speleomantes.Scientific data · 2021Article
- Elemental iron modifies the redox environment of the gastrointestinal tract: A novel therapeutic target and test for metabolic syndrome.Free radical biology & medicine · 2021Article
- Article
- The Protective and Long-Lasting Effects of Human Milk Oligosaccharides on Cognition in Mammals.Nutrients · 2020Review
- The post hoc measurement as a safe and reliable method to age and size plethodontid salamanders.Ecology and evolution · 2020Article
- Photographic database of the European cave salamanders, genus Hydromantes.Scientific data · 2020Article
- Dominant and Subordinate Relationship Formed by Repeated Social Encounters Alters Gut Microbiota in Greater Long-Tailed Hamsters.Microbial ecology · 2020Article
- Into the wild: microbiome transplant studies need broader ecological reality.Proceedings. Biological sciences · 2020Article
- Interactions between Intestinal Microbiota and Host Immune Response in Inflammatory Bowel Disease.Frontiers in immunology · 2017Review
- Serotonin Transporter Genotype Modulates the Gut Microbiota Composition in Young Rats, an Effect Augmented by Early Life Stress.Frontiers in cellular neuroscience · 2017Article
- Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAccumulating evidence indicates interactions between human milk composition, particularly sugars (human milk oligosaccharides or HMO), the gut microbiota of human infants, and behavioral effects. Some HMO secreted in human milk are unable to be endogenously digested by the human infant but are able to be metabolized by certain species of gut microbiota, including
methodsThis study was designed to test two methods, active and passive, of solution administration to mice and the effects on their gut microbiome. Neonatal C57BL/6J mice housed in a specific-pathogen free facility received increasing doses of fructooligosaccharide (FOS) solution or deionized, distilled water. Gastrointestinal (GI) tracts were collected from five dams, six sires, and 41 pups over four time points. Seven fecal pellets from unhandled pups and two pellets from unhandled dams were also collected. Qualitative real-time polymerase chain reaction (qRT-PCR) was used to quantify and compare the amount of
resultsOur results demonstrate a significant difference between the amount of Firmicutes in pups receiving water passively and those receiving FOS actively ( DISCUSSION: From our results, we conclude even handling pups for experimental purposes, without gavage, may induce enough stress to alter the murine gut microbiota profile. We suggest further studies to examine potential stress effects on gut microbiota caused by experimental techniques. Stress from experimental techniques may need to be accounted for in future gut microbiota studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.