ArticleBiomaterials research2017
Study of myoblast differentiation using multi-dimensional scaffolds consisting of nano and micropatterns.
Article in Biomaterials research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Hybrid SES-MEW Scaffold Strategies: A Narrative Review of Multi-Scale Fiber Architectures for Soft and Hard Tissue Engineering.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Biopolymer-Based Electrospun Nanofibers for Wound Healing, Regeneration, and Therapeutics.Materials (Basel, Switzerland) · 2026Review
- In Vitro Fish Cell Culture: From Primary Muscle Cells to Cell-Based Meat in Cyprinidae.Biology · 2026Review
- Bioinspired hydrogel-based regenerative engineering strategies for the structural and functional regeneration of skeletal muscles following volumetric muscle loss.Regenerative biomaterials · 2026Review
- Fabrication and Characterization of Electrospun PCL/GelMA Composite Scaffolds for Muscle Tissue Engineering.Tissue engineering and regenerative medicine · 2025Article
- Functionalized Multi-Walled Carbon Nanotube Enhanced Myogenic Differentiation for Aligned Topography-Induced Skeletal Muscle Engineering.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Decellularized Extracellular Matrix-Derived Hydrogels: a Powerful Class of Biomaterials for Skeletal Muscle Regenerative Engineering Applications.Regenerative engineering and translational medicine · 2025Article
- Mechanical Stimulation and Aligned Poly(ε-caprolactone)-Gelatin Electrospun Scaffolds Promote Skeletal Muscle Regeneration.ACS applied bio materials · 2024Article
- Engineered muscle from micro-channeled PEG scaffold with magnetic FeMaterials today. Bio · 2023Article
- Esophageal wound healing by aligned smooth muscle cell-laden nanofibrous patch.Materials today. Bio · 2023Article
- Triphasic 3D In Vitro Model of Bone-Tendon-Muscle Interfaces to Study Their Regeneration.Cells · 2023Article
- Random cellulose acetate nanofibers: a breakthrough for cultivated meat production.Frontiers in nutrition · 2023Article
- Assessing Edible Filamentous Fungal Carriers as Cell Supports for Growth of Yeast and Cultivated Meat.Foods (Basel, Switzerland) · 2022Article
- Rational design of biodegradable thermoplastic polyurethanes for tissue repair.Bioactive materials · 2022Review
- Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering.ACS biomaterials science & engineering · 2022Review
- Enhanced osteogenic differentiation of alendronate-conjugated nanodiamonds for potential osteoporosis treatment.Biomaterials research · 2021Article
- Nanopattern surface improves cultured human myotube maturation.Skeletal muscle · 2021Article
- Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel.Materials (Basel, Switzerland) · 2021Article
- Graft alignment impacts the regenerative response of skeletal muscle after volumetric muscle loss in a rat model.Acta biomaterialia · 2020Article
- Microcarriers for Upscaling Cultured Meat Production.Frontiers in nutrition · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe topographical cue is major influence on skeletal muscle cell culture because the structure is highly organized and consists of long parallel bundles of multinucleated myotubes that are formed by differentiation and fusion of myoblast satellite cells. In this technical report, we fabricated a multiscale scaffold using electrospinning and poly (ethylene glycol) (PEG) hydrogel micropatterns to monitor the cell behaviors on nano- and micro-alignment combined scaffolds with different combinations of angles.
resultsWe fabricated multiscale scaffolds that provide biocompatible and extracellular matrix (ECM)-mimetic environments via electrospun nanofiber and PEG hydrogel micro patterning. MTT assays demonstrated an almost four-fold increase in the proliferation rate during the 7 days of cell culture for all of the experimental groups. Cell orientation and elongation were measured to confirm the myogenic potential. On the aligned fibrous scaffolds, more than 90% of the cells were dispersed ± 20° of the fiber orientation. To determine cell elongation, we monitored nuclei aspect ratios. On a random nanofiber, the cells demonstrated an aspect ratio of 1.33, but on perpendicular and parallel nanofibers, the aspect ratio was greater than 2. Myosin heavy chain (MHC) expression was significantly higher i) on parallel compared to random fibers, ii) the 100 μm compared to the 200 μm line pattern. We confirmed the disparate trends of myotube formation that can be provoked through multi-dimensional scaffolds.
conclusionWe studied more favorable environments that induce cell alignment and elongation for myogenesis by combining nano- and micro-scale patterns. The fabricated system can serve as a novel multi-dimensional platform to study in vitro cell behaviors.
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