Evidence map›Paper›PMID 28096386›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2017

Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window.

Shoujun Zhu, Qinglai Yang, Alexander L Antaris, Jingying Yue, Zhuoran Ma, Huasen Wang, Wei Huang, Hao Wan, Joy Wang, Shuo Diao and 8 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed, 1 pooled it
19.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 1 synthesis or guideline pooled it, 270 citations in OpenAlex.

  1. Molecular fluorophores for in vivo bioimaging in the second near-infrared window.European journal of nuclear medicine and molecular imaging · 2022
    Pooled it
  2. Article
  3. Article
  4. Review
  5. Materials today. Bio · 2026
    Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Review

19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 3 institutions in 2 countries.

Shoujun ZhuDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Qinglai YangDepartment of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen 518055, China.
Alexander L AntarisDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Jingying YueDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Zhuoran MaDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Huasen WangDepartment of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen 518055, China.
Wei HuangDepartment of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen 518055, China.
Hao WanDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Joy WangDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Shuo DiaoDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Bo ZhangDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Xiaoyang LiDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Yeteng ZhongDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Kuai YuDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Guosong HongDepartment of Chemistry, Stanford University, Stanford, CA 94305.
Jian LuoDepartment of Neurology and Neurological Sciences, School of Medicine, Stanford University, Stanford, CA 94305.
Yongye LiangDepartment of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen 518055, China; liangyy@sustc.edu.cn hdai@stanford.edu.
Hongjie DaiDepartment of Chemistry, Stanford University, Stanford, CA 94305; liangyy@sustc.edu.cn hdai@stanford.edu.
Stanford University · USSouthern University of Science and Technology · CNVA Palo Alto Health Care System · US

Funding

Aligned Nanofibrillar Scaffolds Enhance Angiogenesis and Viability in Ischemia: Sex Differences Administrative SupplementR01HL127113 · NHLBI · STANFORD UNIVERSITY · PI HUANG, NGAN F. · 2016 to 2020
$2.5M
NHLBI NIH HHS R01 HL127113
6 · The paper itself

Abstract

Fluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-infrared (NIR-I; ∼700-900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000-1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ∼1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ∼170-μm histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II windows (800-1,700 nm).

Indexed as

Brain ChemistryClick ChemistryAnimalsBiotinylationBrainCarcinoma, Squamous CellCetuximabFluorescent Antibody Technique, DirectFluorescent DyesImaging, Three-DimensionalMiceMice, Inbred BALB CMice, NudeMicroscopy, ConfocalMolecular StructureNanotubesCetuximabFluorescent DyesStreptavidinbioconjugateclickable dyedensity gradient ultracentrifugation separationNIR-II molecular imagingNIR-II multicolor molecular imaging

Identifiers

PMID28096386
PMCPMC5293099
OpenAlexW2579122533

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.