ArticleProceedings of the National Academy of Sciences of the United States of America2017
Molecular mechanism underlying juvenile hormone-mediated repression of precocious larval-adult metamorphosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 1 of them a synthesis that pooled it.
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Who cites it
83 citing papers in PubMed, 1 synthesis or guideline pooled it, 165 citations in OpenAlex.
- Advances in the study of juvenile hormone signaling.Frontiers in physiology · 2026Pooled it
- Duplicated paralog of insulin receptor functions distinctively in locust reproduction for high fecundity.Insect science · 2026Article
- NFYB Integrates Hormonal Signals into Tissue Allometry by Promoting Protein Biosynthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A self-attention-based deep learning model for identifying key genes in insect pupal metamorphosis.BMC genomics · 2026Article
- Prioritising research on endocrine disruption in the marine environment: a global perspective.Biological reviews of the Cambridge Philosophical Society · 2026Review
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- Integrated In Silico, In Vivo, and Deep Learning Approaches in the Discovery of Novel Candidate Molecules for Aedes aegypti Control.Archives of insect biochemistry and physiology · 2026Article
- Histone acetylation homeodynamics navigates cell survival and apoptosis.Nature communications · 2025Article
- Chinmo function in cockroaches provides new insights into the regulation and evolution of insect metamorphosis.PLoS genetics · 2025Article
- Article
- Developmental changes in the Aedes aegypti mosquito endocrine gland complex.Cell and tissue research · 2025Article
- SUMOylation modulates the dual functions of Krüppel homolog 1 in transcriptional regulation of Broad-Complex expression.Journal of advanced research · 2025Article
- 20E-inducedProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chromatin Accessibility Dynamics Reveal Conserved Transcriptional Regulatory Networks During Insect Metamorphosis inBiology · 2025Article
- Molecular Characterization and Expression of the Ecdysone Receptor and Ultraspiracle Genes in the Wheat Blossom Midge,Insects · 2025Article
- Krüppel homolog 1, a juvenile hormone transcription factor, regulates the reproduction and development of Aedes albopictus(Skuse) (Diptera: Culicidae).Parasitology research · 2025Article
- Article
- Chronologically inappropriate morphogenesis (Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab,International journal of molecular sciences · 2024Article
- Impact of plant monoterpenes on insect pest management and insect-associated microbes.Heliyon · 2024Review
23 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Juvenile hormone (JH) represses precocious metamorphosis of larval to pupal and adult transitions in holometabolous insects. The early JH-inducible gene Krüppel homolog 1 (Kr-h1) plays a key role in the repression of metamorphosis as a mediator of JH action. Previous studies demonstrated that Kr-h1 inhibits precocious larval-pupal transition in immature larva via direct transcriptional repression of the pupal specifier Broad-Complex (BR-C). JH was recently reported to repress the adult specifier gene Ecdysone-induced protein 93F (E93); however, its mechanism of action remains unclear. Here, we found that JH suppressed ecdysone-inducible E93 expression in the epidermis of the silkworm Bombyx mori and in a B. mori cell line. Reporter assays in the cell line revealed that the JH-dependent suppression was mediated by Kr-h1. Genome-wide ChIP-seq analysis identified a consensus Kr-h1 binding site (KBS, 14 bp) located in the E93 promoter region, and EMSA confirmed that Kr-h1 directly binds to the KBS. Moreover, we identified a C-terminal conserved domain in Kr-h1 essential for the transcriptional repression of E93 Based on these results, we propose a mechanism in which JH-inducible Kr-h1 directly binds to the KBS site upstream of the E93 locus to repress its transcription in a cell-autonomous manner, thereby preventing larva from bypassing the pupal stage and progressing to precocious adult development. These findings help to elucidate the molecular mechanisms regulating the metamorphic genetic network, including the functional significance of Kr-h1, BR-C, and E93 in holometabolous insect metamorphosis.
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