ArticleDevelopment (Cambridge, England)2017
High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration.
Article in Development (Cambridge, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- CANTAC-seq analysis reveals E2f1 and Otx1 coordinate zygotic genome activation in Xenopus tropicalis.Nature communications · 2025Article
- A new atlas to study embryonic cell types in Xenopus.Developmental biology · 2024Article
- The journey of a generation: advances and promises in the study of primordial germ cell migration.Development (Cambridge, England) · 2024Article
- Solubility phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition.Developmental cell · 2023Article
- Germline stem cells in human.Signal transduction and targeted therapy · 2022Review
- Comparison of RNA localization during oogenesis withinFrontiers in cell and developmental biology · 2022Article
- Conserved germ plasm characteristics across the Danio and Devario lineages.Genesis (New York, N.Y. : 2000) · 2021Article
- Spatio-temporal mRNA tracking in the early zebrafish embryo.Nature communications · 2021Article
- Molecular asymmetry in the cephalochordate embryo revealed by single-blastomere transcriptome profiling.PLoS genetics · 2020Article
- Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation.Cell reports · 2019Article
- A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification.Developmental cell · 2018Article
- Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan.Scientific reports · 2018Article
- RNA helicase Mov10 is essential for gastrulation and central nervous system development.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- A novel role forDevelopment (Cambridge, England) · 2018Article
- Maternal messages to live by: a personal historical perspective.Genesis (New York, N.Y. : 2000) · 2017Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
During oogenesis, hundreds of maternal RNAs are selectively localized to the animal or vegetal pole, including determinants of somatic and germline fates. Although microarray analysis has identified localized determinants, it is not comprehensive and is limited to known transcripts. Here, we utilized high-throughput RNA-sequencing analysis to comprehensively interrogate animal and vegetal pole RNAs in the fully grown Xenopus laevis oocyte. We identified 411 (198 annotated) and 27 (15 annotated) enriched mRNAs at the vegetal and animal pole, respectively. Ninety were novel mRNAs over 4-fold enriched at the vegetal pole and six were over 10-fold enriched at the animal pole. Unlike mRNAs, microRNAs were not asymmetrically distributed. Whole-mount in situ hybridization confirmed that all 17 selected mRNAs were localized. Biological function and network analysis of vegetally enriched transcripts identified protein-modifying enzymes, receptors, ligands, RNA-binding proteins, transcription factors and co-factors with five defining hubs linking 47 genes in a network. Initial functional studies of maternal vegetally localized mRNAs show that sox7 plays a novel and important role in primordial germ cell (PGC) development and that ephrinB1 (efnb1) is required for proper PGC migration. We propose potential pathways operating at the vegetal pole that highlight where future investigations might be most fruitful.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.