ArticleBioconjugate chemistry2017
Effect of the Protein Corona on Antibody-Antigen Binding in Nanoparticle Sandwich Immunoassays.
Article in Bioconjugate chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 71 citations in OpenAlex.
- Reverse-Engineered Lipid Nanoparticles Harness Protein Corona Formation for Cytokine Sequestration and Combination Immunomodulatory Therapy.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Catch-and-display immunoassay for digital biomarker detection.Nanoscale horizons · 2026Article
- Article
- Loading Density Influences the Tumor Cell Targeting and Signaling Inhibition Capabilities of Antibody Nanoconjugates.ACS omega · 2026Article
- Catch-and-Display Immunoassay as an Accessible Platform for Digital Biomarker Detection.bioRxiv : the preprint server for biology · 2026Article
- Development of an Immunoassay for Highly Pathogenic Avian Influenza (H5N1) across Diverse Sample Matrices.ACS nanoscience Au · 2025Article
- Overcoming Limited Access to Virus Infection Rapid Testing: Development of a Lateral Flow Test for SARS-CoV-2 with Locally Available Resources.Biosensors · 2024Article
- Gold Nanoparticle Paper Immunoassays for Sensing the Presence ofACS omega · 2023Article
- Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics.Beilstein journal of nanotechnology · 2023Review
- The Influence of Preforming Protein Coronas on the Performance of Dengue NS1 Immunoassays.Pharmaceutics · 2022Article
- Nanomaterial and interface advances in immunoassay biosensors.The journal of physical chemistry. C, Nanomaterials and interfaces · 2022Article
- Designing Functional Bionanoconstructs for EffectiveBioconjugate chemistry · 2022Review
- Fluorescent paper strip immunoassay with carbon nanodots@silica for determination of human serum amyloid A1.Mikrochimica acta · 2021Article
- Inhibition of Wnt signaling by Frizzled7 antibody-coated nanoshells sensitizes triple-negative breast cancer cells to the autophagy regulator chloroquine.Nano research · 2020Article
- Designing Paper-Based Immunoassays for Biomedical Applications.Sensors (Basel, Switzerland) · 2019Review
- Reporter Selection for Nanotags in Multiplexed Surface Enhanced Raman Spectroscopy Assays.ACS omega · 2018Article
- Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1.Biomicrofluidics · 2018Article
- Challenges of the Nano-Bio Interface in Lateral Flow and Dipstick Immunoassays.Trends in biotechnology · 2017Review
- Surface-Enhanced Raman Spectroscopy-Based Sandwich Immunoassays for Multiplexed Detection of Zika and Dengue Viral Biomarkers.ACS infectious diseases · 2017Article
- Design of SERS nanotags for multiplexed lateral flow immunoassays.Molecular systems design & engineering · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
We investigated the effect of the protein corona on the function of nanoparticle (NP) antibody (Ab) conjugates in dipstick sandwich immunoassays. Ab specific for Zika virus nonstructural protein 1 (NS1) were conjugated to gold NPs, and another anti-NS1 Ab was immobilized onto the nitrocellulose membrane. Sandwich immunoassay formation was influenced by whether the strip was run in corona forming conditions, i.e., in human serum. Strips run in buffer or pure solutions of bovine serum albumin exhibited false positives, but those run in human serum did not. Serum pretreatment of the nitrocellulose also eliminated false positives. Corona formation around the NP-Ab in serum was faster than the immunoassay time scale. Langmuir binding analysis determined how the immobilized Ab affinity for the NP-Ab/NS1 was impacted by corona formation conditions, quantified as an effective dissociation constant, K
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.