ArticleScientific reports2017
Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- TRPA1 for Butterfly Eyespot Formation.International journal of molecular sciences · 2026Article
- Wound-induced eyespots on butterfly wings at the intersection of immune response and pigmentation development.BMC biology · 2025Article
- Article
- Article
- Eyespot variation and field temperature in the Meadow Brown butterfly.Ecology and evolution · 2024Article
- Article
- Protein Delivery to Insect Epithelial Cells In Vivo: Potential Application to Functional Molecular Analysis of Proteins in Butterfly Wing Development.Biotech (Basel (Switzerland)) · 2023Article
- Article
- Article
- Speciation in North AmericanSystematic entomology · 2020Article
- Butterfly eyespot color pattern formation requires physical contact of the pupal wing epithelium with extracellular materials for morphogenic signal propagation.BMC developmental biology · 2020Article
- Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue ButterflyJournal of imaging · 2019Article
- Article
- Developmental dynamics of butterfly wings: real-time in vivo whole-wing imaging of twelve butterfly species.Scientific reports · 2018Article
- Emperors, admirals and giants, zebras, tigers and woolly bears: casting a broader net in exploring heparin effects on Lepidoptera wing patterns.F1000Research · 2018Article
- Wound healing, calcium signaling, and other novel pathways are associated with the formation of butterfly eyespots.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Butterfly wing eyespot patterns are determined in pupal tissues by organisers located at the centre of the prospective eyespots. Nevertheless, organiser cells have not been examined cytochemically in vivo, partly due to technical difficulties. Here, we directly observed organiser cells in pupal forewing epithelium via an in vivo confocal fluorescent imaging technique, using 1-h post-pupation pupae of the blue pansy butterfly, Junonia orithya. The prospective eyespot centre was indented from the plane of the ventral tissue surface. Three-dimensional reconstruction images revealed that the apical portion of "focal cells" at the bottom of the eyespot indentation contained many mitochondria. The mitochondrial portion was connected with a "cell body" containing a nucleus. Most focal cells had globular nuclei and were vertically elongated, but cells in the wing basal region had flattened nuclei and were tilted toward the distal direction. Epithelial cells in any wing region had cytoneme-like horizontal processes. From 1 h to 10 h post-pupation, nuclear volume increased, suggesting DNA synthesis during this period. Morphological differences among cells in different regions may suggest that organiser cells are developmentally ahead of cells in other regions and that position-dependent heterochronic development is a general mechanism for constructing colour patterns in butterfly wings.
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