Evidence map›Paper›PMID 28094784›Full record

ArticleNature communications2017

Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy.

Malte Kühnemund, Qingshan Wei, Evangelia Darai, Yingjie Wang, Iván Hernández-Neuta, Zhao Yang, Derek Tseng, Annika Ahlford, Lucy Mathot, Tobias Sjöblom and 2 more

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
9.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 134 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Next-Generation Microfluidics for Biomedical Research and Healthcare Applications.Biomedical engineering and computational biology · 2023
    Review
  9. Article
  10. Article
  11. Review
  12. Lab-on-a-Chip Devices for Point-of-Care Medical Diagnostics.Advances in biochemical engineering/biotechnology · 2022
    Review
  13. Review
  14. A Smartphone-Enabled Portable Digital Light Processing 3D Printer.Advanced materials (Deerfield Beach, Fla.) · 2021
    Article
  15. Review
  16. Article
  17. Article
  18. Review
  19. Electrochemical Genosensing ofSensors (Basel, Switzerland) · 2021
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Malte KühnemundScience for Life Laboratory, Department of Immunology, genetics and pathology, Uppsala University, Box 815, 751 08 Uppsala, Sweden.
Qingshan WeiElectrical Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
Evangelia DaraiScience for Life Laboratory, Department of Biophysics and biochemistry, Stockholm University, Sweden, Box 1031, Se-171 21 Solna, Sweden.
Yingjie WangElectrical Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
Iván Hernández-NeutaScience for Life Laboratory, Department of Biophysics and biochemistry, Stockholm University, Sweden, Box 1031, Se-171 21 Solna, Sweden.
Zhao YangElectrical Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
Derek TsengElectrical Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
Annika AhlfordScience for Life Laboratory, Department of Biophysics and biochemistry, Stockholm University, Sweden, Box 1031, Se-171 21 Solna, Sweden.
Lucy MathotScience for Life Laboratory, Department of Immunology, genetics and pathology, Uppsala University, Box 815, 751 08 Uppsala, Sweden.
Tobias SjöblomScience for Life Laboratory, Department of Immunology, genetics and pathology, Uppsala University, Box 815, 751 08 Uppsala, Sweden.
Aydogan OzcanElectrical Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
Mats NilssonScience for Life Laboratory, Department of Immunology, genetics and pathology, Uppsala University, Box 815, 751 08 Uppsala, Sweden.
Stockholm University · SEUniversity of California, Los Angeles · USScience for Life Laboratory · SECalifornia NanoSystems Institute · USUppsala University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies.

Indexed as

Cell PhoneDNAHumansMicroscopyMutationNeoplasmsPathology, MolecularProto-Oncogene Proteins p21(ras)Sequence Analysis, DNADNAKRAS protein, humanProto-Oncogene Proteins p21(ras)

Identifiers

PMID28094784
PMCPMC5247573
OpenAlexW2574986173

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.