Evidence map›Paper›PMID 28094782›Full record

ArticleScientific reports2017

Cocaine Enhances DC to T-cell HIV-1 Transmission by Activating DC-SIGN/LARG/LSP1 Complex and Facilitating Infectious Synapse Formation.

Anil Prasad, Rutuja Kulkarni, Shuxian Jiang, Jerome E Groopman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. LSP1-myosin1e bimolecular complex regulates focal adhesion dynamics and cell migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
  4. Article
  5. Review
  6. Antigen structure affects cellular routing through DC-SIGN.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  7. Review
  8. Review
  9. An atypical subacute presentation of posterior reversible encephalopathy syndrome.Journal of community hospital internal medicine perspectives · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Anil PrasadDivision of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Rutuja KulkarniDivision of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Shuxian JiangDivision of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Jerome E GroopmanDivision of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Harvard University · USBeth Israel Deaconess Medical Center · US

Funding

Novel Strategies to Antagonize Cocaine-Enhanced HIV PathobiologyR01DA036298 · NIDA · BETH ISRAEL DEACONESS MEDICAL CENTER · PI GROOPMAN, JEROME E, MEMPEL, THORSTEN ROMAN · 2013 to 2017
$2.0M
Novel Mechanisms of Disarming Anti-HIV Host Defenses by MethamphetamineR01DA039566 · NIDA · BETH ISRAEL DEACONESS MEDICAL CENTER · PI GROOPMAN, JEROME E · 2015 to 2019
$1.8M
NIDA NIH HHS R01 DA036298NIDA NIH HHS R01 DA039566
6 · The paper itself

Abstract

DC-SIGN is a dendritic cell surface structure which participates in binding and transmission of HIV-1. Here, for the first time we demonstrate that cocaine induces over expression of DC-SIGN and significantly enhances virus transfer from DCs to T-cells by increasing the binding and internalization of HIV-1 in DCs. We found that cocaine activates a DC-SIGN mediated 'signalosome' complex by enhancing its association with LARG and LSP1. Further, LARG was observed to participate in DC-SIGN mediated internalization of HIV-1 in DCs. Intracellular trafficking studies of HIV-1 in cocaine treated DCs revealed increased co-localization of HIV-1 with endosomal or multi vesicular body (MVB) markers such as CD81 and VPS4 and decreased co-localization with the phagolysomal marker LAMP1; this signified altered intracellular trafficking and decreased degradation of HIV-1 in cocaine treated DCs. Furthermore, we found that cocaine induced activation of LARG which in turn activated Rho A and the focal adhesion molecules FAK, Pyk2 and paxillin. This signaling cascade enhanced the formation of an infectious synapse between DCs and T-cells. Our study provides insight into the molecular mechanisms of cocaine's contribution to key components in HIV pathogenesis and highlights novel targets for interrupting the virus life cycle in substance using hosts.

Indexed as

ATPases Associated with Diverse Cellular ActivitiesBiological TransportCD4-Positive T-LymphocytesCell Adhesion MoleculesCocaineDC-Specific ICAM-3 Grabbing NonintegrinDendritic CellsEndosomal Sorting Complexes Required for TransportFocal AdhesionsGene ExpressionHIV-1HIV InfectionsHost-Pathogen InteractionsHumansImmunological SynapsesLectins, C-TypeARHGEF12 protein, humanATPases Associated with Diverse Cellular ActivitiesCell Adhesion MoleculesCocaineDC-Specific ICAM-3 Grabbing NonintegrinEndosomal Sorting Complexes Required for TransportLAMP1 protein, humanLectins, C-TypeLSP1 protein, humanLysosomal-Associated Membrane Protein 1Lysosomal Membrane ProteinsMicrofilament ProteinsReceptors, CCR5Receptors, Cell SurfaceReceptors, CXCR4rhoA GTP-Binding ProteinRho Guanine Nucleotide Exchange FactorsTetraspanin 28Vacuolar Proton-Translocating ATPasesVPS4A protein, human

Identifiers

PMID28094782
PMCPMC5240552
OpenAlexW2571734993

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.