ArticleScientific reports2017
Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- Mixed-Pathogen Infections in Vegetatively Propagated Crops: From Biological Synergism to Integrated Management.Plants (Basel, Switzerland) · 2026Review
- Biomolecule Detection Methods Based on Nanoparticle Approaches.ACS omega · 2026Review
- Electrochemical Biosensing Platforms for Rapid and Early Diagnosis of Crop Fungal and Viral Diseases.Sensors (Basel, Switzerland) · 2026Review
- Advanced Spectroscopic, Imaging, and Nanotechnology Tools for Diagnosing Fungal Diseases in Fruits.Food science & nutrition · 2026Review
- Review
- Electrochemical Biosensors for Oilseed Crops: Nanomaterial-Driven Detection and Smart Agriculture.Foods (Basel, Switzerland) · 2025Review
- Agricultural nanotechnology for a safe and sustainable future: current status, challenges, and beyond.Journal of the science of food and agriculture · 2025Review
- Can nanotechnology and genomics innovations trigger agricultural revolution and sustainable development?Functional & integrative genomics · 2024Review
- Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems.3 Biotech · 2024Review
- Revisiting the Role of Sensors for Shaping Plant Research: Applications and Future Perspectives.Sensors (Basel, Switzerland) · 2024Review
- A Single Electrochemical Biosensor Designed to Detect Any Virus.Analytical chemistry · 2024Article
- Nanotechnology in Food and Plant Science: Challenges and Future Prospects.Plants (Basel, Switzerland) · 2023Review
- Scope of Onsite, Portable Prevention Diagnostic Strategies forBiosensors · 2023Review
- Development of a Novel Phagomagnetic-Assisted Isothermal DNA Amplification System for Endpoint Electrochemical Detection ofBiosensors · 2023Article
- Optical Sensing Technologies to Elucidate the Interplay between Plant and Microbes.Micromachines · 2023Review
- Current and emerging trends in techniques for plant pathogen detection.Frontiers in plant science · 2023Review
- Review
- Review
- Nanosensor Applications in Plant Science.Biosensors · 2022Review
- Nanomaterials coupled with microRNAs for alleviating plant stress: a new opening towards sustainable agriculture.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developing quick and sensitive molecular diagnostics for plant pathogen detection is challenging. Herein, a nanoparticle based electrochemical biosensor was developed for rapid and sensitive detection of plant pathogen DNA on disposable screen-printed carbon electrodes. This 60 min assay relied on the rapid isothermal amplification of target pathogen DNA sequences by recombinase polymerase amplification (RPA) followed by gold nanoparticle-based electrochemical assessment with differential pulse voltammetry (DPV). Our method was 10,000 times more sensitive than conventional polymerase chain reaction (PCR)/gel electrophoresis and could readily identify P. syringae infected plant samples even before the disease symptoms were visible. On the basis of the speed, sensitivity, simplicity and portability of the approach, we believe the method has potential as a rapid disease management solution for applications in agriculture diagnostics.
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Registered trials
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