Evidence map›Paper›PMID 28093887›Full record

ArticleJournal of biomedical materials research. Part A2017

Collagen hydrogel scaffold promotes mesenchymal stem cell and endothelial cell coculture for bone tissue engineering.

Bao-Ngoc B Nguyen, Rebecca A Moriarty, Tim Kamalitdinov, Julie M Etheridge, John P Fisher

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical materials research. Part A, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 86 citations in OpenAlex.

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  16. [Effect of fibroblasts on promoting the sprout and migration of endothelial cells in three-dimensional pre-vascularized microstructures].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Bao-Ngoc B NguyenFischell Department of Bioengineering, University of Maryland, College Park, Maryland, 20742.
Rebecca A MoriartyFischell Department of Bioengineering, University of Maryland, College Park, Maryland, 20742.
Tim KamalitdinovFischell Department of Bioengineering, University of Maryland, College Park, Maryland, 20742.
Julie M EtheridgeFischell Department of Bioengineering, University of Maryland, College Park, Maryland, 20742.
John P FisherFischell Department of Bioengineering, University of Maryland, College Park, Maryland, 20742.
University of Maryland, College Park · US

Funding

Application of Tubular Perfusion System (TPS) Generated Prevascularized Bone TissR01AR061460 · NIAMS · UNIV OF MARYLAND, COLLEGE PARK · PI FISHER, JOHN P · 2011 to 2014
$1.3M
NIAMS NIH HHS R01 AR061460
6 · The paper itself

Abstract

The generation of functional, vascularized tissues is a key challenge for the field of tissue engineering. Before clinical implantations of such tissue engineered bone constructs can succeed, tactics to promote neovascularization need to be strengthened. We have previously demonstrated that the tubular perfusion system (TPS) bioreactor is an effective culturing method to augment osteogenic differentiation and maintain viability of human mesenchymal stem cells (hMSC). Here, we devised a strategy to address the need for a functional microvasculature by designing an in vitro coculture system that simultaneously cultures osteogenic differentiating hMSCs with endothelial cells (ECs). We utilized the TPS bioreactor as a dynamic coculture environment, which we hypothesize will encourage prevascularization of endothelial cells and early formation of bone tissue and could aid in anastomosis of the graft with the host vasculature after patient implantation. To evaluate the effect of different natural scaffolds for this coculture system, the cells were encapsulated in alginate and/or collagen hydrogel scaffolds. We discovered the necessity of cell-to-cell proximity between the two cell types as well as preference for the natural cell binding capabilities of hydrogels like collagen. We discovered increased osteogenic and angiogenic potential as seen by amplified gene and protein expression of ALP, BMP-2, VEGF, and PECAM. The TPS bioreactor further augmented these expressions, indicating a synergistic effect between coculture and applied shear stress. The development of this dynamic coculture platform for the prevascularization of engineered bone, emphasizing the importance of the construct microenvironments and will advance the clinical use of tissue engineered constructs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1123-1131, 2017.

Indexed as

Neovascularization, PhysiologicOsteogenesisBioreactorsCoculture TechniquesCollagenEndothelial CellsHumansHydrogelsMesenchymal Stem CellsTissue ScaffoldsCollagenHydrogelsbone regenerationcocultureendothelial cellshydrogel scaffoldmesenchymal stem cellsTPS bioreactor

Identifiers

PMID28093887
PMCPMC5328802
OpenAlexW2578602994

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.