ArticleNature biotechnology2017
Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism.
Article in Nature biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 155 papers.
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Who cites it
155 citing papers in PubMed, 475 citations in OpenAlex.
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- Improvement of the production of biomass and lipid by Papiliotrema laurentii combining flux balance analysis and central composite rotational design.Bioprocess and biosystems engineering · 2026Article
- Engineering Modification for the Efficient Synthesis of β-ionone by Yarrowia lipolytica.Biotechnology journal · 2026Article
- Whole-Cell Biocatalysis for the Production of Structurally Diverse Methoxydihydrochalcones: Broad Activity of theMolecules (Basel, Switzerland) · 2026Article
- Acetyl- and malonyl-CoA availability drive EPA selectivity in polyketide synthase-engineered Yarrowia lipolytica.Microbial cell factories · 2026Article
- Defined YNB-free mineral medium improves reproducibility and enables high-titer production in Yarrowia lipolytica.Microbial cell factories · 2026Article
- Improved de novo production of prostaglandin FCommunications biology · 2026Article
- Lipid accumulation in nitrogen and phosphorus-limited yeast is caused by less growth-related dilution.Metabolic engineering · 2026Article
- EngineeringSynthetic and systems biotechnology · 2025Article
- Suppressing Glycerol-3-phosphate Phosphatase and Enhancing Glycerol-3-Phosphate Shuttle Flux Crucial for High-Efficiency Fatty Acid Production in the Fast-Growing Oleaginous Schizochytrium.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Xylose Metabolism Perturbation in Yarrowia lipolytica for Efficient Succinic Acid Bioproduction from Lignocellulosic Biomass.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- In Vivo DNA Assembly inACS synthetic biology · 2025Article
- Enhancing abscisic acid production inSynthetic and systems biotechnology · 2025Article
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Metabolic Engineering ofACS engineering Au · 2025Article
- The role of ATP citrate lyase, phosphoketolase, and malic enzyme in oleaginous Rhodotorula toruloides.Applied microbiology and biotechnology · 2025Article
- Reprogramming yeast metabolism for customized starch-rich micro-grain through low-carbon microbial manufacturing.Nature communications · 2025Article
- Novel cell factory for the production of 24-epi-ergosterol, an un-natural semi-synthetic precursor for the production of brassinolide in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2025Article
95 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial factories have been engineered to produce lipids from carbohydrate feedstocks for production of biofuels and oleochemicals. However, even the best yields obtained to date are insufficient for commercial lipid production. To maximize the capture of electrons generated from substrate catabolism and thus increase substrate-to-product yields, we engineered 13 strains of Yarrowia lipolytica with synthetic pathways converting glycolytic NADH into the lipid biosynthetic precursors NADPH or acetyl-CoA. A quantitative model was established and identified the yield of the lipid pathway as a crucial determinant of overall process yield. The best engineered strain achieved a productivity of 1.2 g/L/h and a process yield of 0.27 g-fatty acid methyl esters/g-glucose, which constitutes a 25% improvement over previously engineered yeast strains. Oxygen requirements of our highest producer were reduced owing to decreased NADH oxidization by aerobic respiration. We show that redox engineering could enable commercialization of microbial carbohydrate-based lipid production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.