ArticleScientific reports2017
Protein crystal nucleation in pores.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Investigating the Effects of Nanogels in Promoting Protein Crystallization.International journal of molecular sciences · 2026Article
- A phase transition modulates the protective function of a tardigrade disordered protein during desiccation.Protein science : a publication of the Protein Society · 2025Article
- Nucleation of protein crystals in pores and their growth.Biophysical reviews · 2025Review
- Universality of Hair as a Nucleant: Exploring the Effects of Surface Chemistry and Topography.Crystal growth & design · 2023Article
- A workflow for the development of template-assisted membrane crystallization downstream processing for monoclonal antibody purification.Nature protocols · 2023Review
- Heterogeneous Nucleation in Protein Crystallization.Biomimetics (Basel, Switzerland) · 2023Review
- Protein Crystals Nucleated and Grown by Means of Porous Materials Display Improved X-ray Diffraction Quality.International journal of molecular sciences · 2022Article
- Theoretical and experimental investigation of protein crystal nucleation in pores and crevices.IUCrJ · 2021Article
- Crystallisation Behaviour of Pharmaceutical Compounds Confined within Mesoporous Silicon.Pharmaceutics · 2020Review
- Quantum scale biomimicry of low dimensional growth: An unusual complex amorphous precursor route to TiOScientific reports · 2019Article
- Successful sample preparation for serial crystallography experiments.Journal of applied crystallography · 2019Article
- Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser.Scientific reports · 2018Article
- The role of phase separation and related topography in the exceptional ice-nucleating ability of alkali feldspars.Physical chemistry chemical physics : PCCP · 2017Article
- Can the propensity of protein crystallization be increased by using systematic screening with metals?Protein science : a publication of the Protein Society · 2017Review
- Protein crystallization: Eluding the bottleneck of X-ray crystallography.AIMS biophysics · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most powerful method for protein structure determination is X-ray crystallography which relies on the availability of high quality crystals. Obtaining protein crystals is a major bottleneck, and inducing their nucleation is of crucial importance in this field. An effective method to form crystals is to introduce nucleation-inducing heterologous materials into the crystallization solution. Porous materials are exceptionally effective at inducing nucleation. It is shown here that a combined diffusion-adsorption effect can increase protein concentration inside pores, which enables crystal nucleation even under conditions where heterogeneous nucleation on flat surfaces is absent. Provided the pore is sufficiently narrow, protein molecules approach its walls and adsorb more frequently than they can escape. The decrease in the nucleation energy barrier is calculated, exhibiting its quantitative dependence on the confinement space and the energy of interaction with the pore walls. These results provide a detailed explanation of the effectiveness of porous materials for nucleation of protein crystals, and will be useful for optimal design of such materials.
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