Evidence map›Paper›PMID 28090815›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2017

Comparison of Genome-Wide and Gene-Specific DNA Methylation Profiling in First-Trimester Chorionic Villi From Pregnancies Conceived With Infertility Treatments.

Ning Xu, Gillian M Barlow, Jinrui Cui, Erica T Wang, Bora Lee, Marzieh Akhlaghpour, Lindsay Kroener, John Williams, Jerome I Rotter, Yii-der I Chen and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
8.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Epigenetic Risks of Medically Assisted Reproduction.Journal of clinical medicine · 2022
    Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. DNA methylation differences at birth after conception through ART.Human reproduction (Oxford, England) · 2021
    Article
  12. Review
  13. Article
  14. Article
  15. Genetics and Epigenetics of Infertility and Treatments on Outcomes.The Journal of clinical endocrinology and metabolism · 2019
    Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Ning Xu1 Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Gillian M Barlow2 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Jinrui Cui1 Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Erica T Wang2 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Bora Lee2 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Marzieh Akhlaghpour2 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Lindsay Kroener2 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
John Williams3 UCLA School of Medicine, Los Angeles, CA, USA.
Jerome I Rotter5 Institute for Translational Genomics and Population Sciences, LA Biomed/Harbor-UCLA Medical Center, Torrance, CA, USA.
Yii-der I Chen5 Institute for Translational Genomics and Population Sciences, LA Biomed/Harbor-UCLA Medical Center, Torrance, CA, USA.
Mark O Goodarzi1 Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Margareta D Pisarska2 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Cedars-Sinai Medical Center · USUniversity of California, Los Angeles · USHarbor–UCLA Medical Center · USUCLA Medical Center · US

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
Effects of fetal sex on the first trimester transcriptomeR01HD074368 · NICHD · CEDARS-SINAI MEDICAL CENTER · PI PISARSKA, MARGARETA · 2013 to 2018
$3.4M
NCATS NIH HHS UL1 TR001881NICHD NIH HHS R01 HD074368
6 · The paper itself

Abstract

backgroundAssisted reproductive technologies are associated with altered methylation in term placenta. However, it is unclear whether methylation patterns are the result of fertility treatments or intrauterine environment. Thus, we set out to determine whether there are differences in the first-trimester placenta that may be altered by the underlying fertility treatments. Genome-wide DNA methylation analyses from chorionic villus sampling (CVS) from matched singleton pregnancies conceived using in vitro fertilization (IVF), non-IVF fertility treatment (NIFT), or those conceived spontaneously were performed using Illumina Infinium HumanMethylation450 BeadChip from 15 matched CVS samples. Nanofluidic quantitative polymerase chain reaction (qPCR) of differently methylated genes was performed in a confirmatory cohort of 23 IVF conceptions and 24 NIFT conceptions.

resultsGlobal methylation was similar among the IVF, NIFT, and spontaneous conceptions. However, differential methylation from IVF and NIFT pregnancies was present at 34 CpG sites, which was significantly different. Of those, 14 corresponded to known genes, with methylation changes detected at multiple loci in 3 genes, anaphase-promoting complex subunit 2 ( ANAPC2), C-X-C motif chemokine ligand 14 ( CXCL14), and regulating synaptic membrane exocytosis 1 ( RIMS1). Nanofluidic qPCR of differentially methylated genes identified pre T-cell antigen receptor alpha ( PTCRA) to be significantly downregulated in IVF versus NIFT conceptions.

conclusionAlthough global methylation patterns are similar, there are differences in methylation of specific genes in IVF compared to NIFT conceptions, leading to altered gene expression. PTCRA was differentially methylated and downregulated in IVF conceptions, warranting further investigation. It remains to be determined whether these changes affect placentation and whether it is due to the more profound underlying infertility requiring IVF, yet these data provide unique insight into the first-trimester placental epigenome.

Indexed as

DNA MethylationFertilization in VitroAdultApc2 Subunit, Anaphase-Promoting Complex-CyclosomeChemokines, CXCChorionic VilliFemaleGene Expression ProfilingGenetic LociGTP-Binding ProteinsHumansInfertilityMembrane GlycoproteinsNerve Tissue ProteinsPregnancyPregnancy Trimester, FirstANAPC2 protein, humanApc2 Subunit, Anaphase-Promoting Complex-CyclosomeChemokines, CXCCXCL14 protein, humanGTP-Binding ProteinsMembrane GlycoproteinsNerve Tissue Proteinspre-T cell receptor alphaReceptors, Antigen, T-Cell, alpha-betaRIMS1 protein, humanepigeneticsIVFNIFTplacentaPTCRA

Identifiers

PMID28090815
PMCPMC5933085
OpenAlexW2556059189

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.