ArticleClinical and experimental reproductive medicine2016
Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting.
Article in Clinical and experimental reproductive medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Comparative analysis of sperm preparation techniques on DNA fragmentation and clinical outcomes: a network meta-analysis.Frontiers in endocrinology · 2026Pooled it
- Sequencing Matters: Comparative Effectiveness of Density-Gradient Centrifugation and MACS for Enhancing Sperm Quality and DNA Integrity.Journal of clinical medicine · 2026Article
- Integrative functional and molecular characterization of Bali bull semen and its relationship with reproductive performance.Veterinary world · 2026Article
- Magnetic-Activated Cell Sorting as a Method to Improve Necrozoospermia-Related Asthenozoospermic Samples.Journal of clinical medicine · 2022Article
- Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation.Journal of clinical medicine · 2020Article
- PICSI vs. MACS for abnormal sperm DNA fragmentation ICSI cases: a prospective randomized trial.Journal of assisted reproduction and genetics · 2020Article
- Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- ICSI significantly improved the pregnancy rate of patients with a high sperm DNA fragmentation index.Clinical and experimental reproductive medicine · 2017Article
- MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content: a pilot study.Asian journal of andrologyArticle
- Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer.Asian journal of andrologyArticle
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency.
methodsSemen analysis and a sperm DNA fragmentation assay were performed to assess the correlations between semen parameters and the DFI in 458 semen samples. Sperm with progressive motility or non-apoptosis were isolated by DGC or MACS, respectively, in 29 normozoospermic semen samples. The effects of DGC or MACS alone and of DGC and MACS combined on reducing the amount of sperm in the sample with DNA fragmentation and protamine deficiency were investigated.
resultsThe sperm DFI showed a significant correlation (
conclusionThe combination of DGC and MACS may be an effective method to isolate high-quality sperm with progressive motility, non-apoptosis, high DNA integrity, and low protamine deficiency in clinical use.
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