Evidence map›Paper›PMID 28090423›Full record

ArticleTranslation (Austin, Tex.)2016

Fusion proteins of

Elizabeth Levins, Ching-Ying Tseng, Ryan M Patrick, Laura K Mayberry, Nicola Cole, Karen S Browning

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translation (Austin, Tex.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Elizabeth LevinsDepartment of Molecular Biosciences and Institute for Cell and Molecular Biology, University of Texas at Austin , Austin, TX, USA.
Ching-Ying TsengDepartment of Molecular Biosciences and Institute for Cell and Molecular Biology, University of Texas at Austin , Austin, TX, USA.
Ryan M PatrickDepartment of Molecular Biosciences and Institute for Cell and Molecular Biology, University of Texas at Austin , Austin, TX, USA.
Laura K MayberryDepartment of Molecular Biosciences and Institute for Cell and Molecular Biology, University of Texas at Austin , Austin, TX, USA.
Nicola ColeDepartment of Molecular Biosciences and Institute for Cell and Molecular Biology, University of Texas at Austin , Austin, TX, USA.
Karen S BrowningDepartment of Molecular Biosciences and Institute for Cell and Molecular Biology, University of Texas at Austin , Austin, TX, USA.
The University of Texas at Austin · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The use of fluorescent proteins fused to other proteins has been very useful in revealing the location and function of many proteins. However, it is very important to show that the fusion of these reporter proteins does not impact the function of the protein of interest. Plants have 2 forms of the cap-binding protein that function in initiation of translation, eIF4E and a plant specific form, eIFiso4E. In an attempt to determine the cellular localization of eIFiso4E, fusions to GFP were made, but were found to not be competent to rescue the lethal phenotype of plants lacking eIF4E and eIFiso4E. This suggested that the GFP fusions at either the N- or C-terminus of eIFiso4E were not functional. Biochemical analysis of the fusions revealed that eIFiso4E•GFP fusions were not able to bind to m

Indexed as

cap-binding proteineukaryotic initiation factorfusion proteinGFPtranslation

Identifiers

PMID28090423
PMCPMC5173309
OpenAlexW2558595782

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.