Evidence map›Paper›PMID 28089519›Full record

ArticleBiochimica et biophysica acta. Gene regulatory mechanisms2017

Role of Nhp6 and Hmo1 in SWI/SNF occupancy and nucleosome landscape at gene regulatory regions.

Matias I Hepp, Michaela Smolle, Cristian Gidi, Roberto Amigo, Nicole Valenzuela, Axel Arriagada, Alejandro Maureira, Madelaine M Gogol, Marcela Torrejón, Jerry L Workman and 1 more

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In one paragraph

Article in Biochimica et biophysica acta. Gene regulatory mechanisms, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Matias I HeppDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile.
Michaela SmolleStowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA.
Cristian GidiDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile.
Roberto AmigoDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile.
Nicole ValenzuelaDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile.
Axel ArriagadaDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile.
Alejandro MaureiraDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile.
Madelaine M GogolStowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA.
Marcela TorrejónDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile.
Jerry L WorkmanStowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA.
José L GutiérrezDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción 4070043, Chile. Electronic address: lgutier@udec.cl.

Funding

Transcription regulation in chromatinR35GM118068 · NIGMS · STOWERS INSTITUTE FOR MEDICAL RESEARCH · PI JERRY L WORKMAN · 2016 to 2026
$4.2M
NIGMS NIH HHS R35 GM118068
6 · The paper itself

Abstract

Diverse chromatin modifiers are involved in regulation of gene expression at the level of transcriptional regulation. Among these modifiers are ATP-dependent chromatin remodelers, where the SWI/SNF complex is the founding member. It has been observed that High Mobility Group (HMG) proteins can influence the activity of a number of these chromatin remodelers. In this context, we have previously demonstrated that the yeast HMG proteins Nhp6 and Hmo1 can stimulate SWI/SNF activity. Here, we studied the genome-wide binding patterns of Nhp6, Hmo1 and the SWI/SNF complex, finding that most of gene promoters presenting high occupancy of this complex also display high enrichment of these HMG proteins. Using deletion mutant strains we demonstrate that binding of SWI/SNF is significantly reduced at numerous genomic locations by deletion of NHP6 and/or deletion of HMO1. Moreover, alterations in the nucleosome landscape take place at gene promoters undergoing reduced SWI/SNF binding. Additional analyses show that these effects also correlate with alterations in transcriptional activity. Our results suggest that, besides the ability to stimulate SWI/SNF activity, these HMG proteins are able to assist the loading of this complex onto gene regulatory regions.

Indexed as

Chromosomal Proteins, Non-HistoneHigh Mobility Group ProteinsHMGN ProteinsNucleosomesRegulatory Sequences, Nucleic AcidSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsChromosomal Proteins, Non-HistoneHigh Mobility Group ProteinsHMGN ProteinsHMO1 protein, S cerevisiaeNHP6A protein, S cerevisiaeNucleosomesSaccharomyces cerevisiae ProteinsSWI-SNF-B chromatin-remodeling complexTranscription FactorsChromatin remodelingHMGHmo1Nhp6SWI/SNF

Identifiers

PMID28089519
PMCPMC5913752

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.