ArticleMolecular nutrition & food research2017
Increasing dietary nitrate has no effect on cancellous bone loss or fecal microbiome in ovariectomized rats.
Article in Molecular nutrition & food research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- The association of nitrate exposure with bone mineral density in adolescents aged 12 to 19: A cross-sectional study.Medicine · 2026Article
- Effect of Supplementation with Red Rooibos Tea on Body Composition, Metabolic Outcomes, and Movement in Ovariectomized Sprague-Dawley Rats.Current developments in nutrition · 2026Article
- The potential role of nitrate, a nitric oxide donor, in the prevention and treatment of diabetic osteoporosis.Frontiers in endocrinology · 2025Review
- Role of oral and gut microbiomes in enterosalivary nitrate metabolism and their effects on systemic disease.Frontiers in cellular and infection microbiology · 2025Review
- The association of dietary nitrates/nitrites intake and the gut microbial metabolite trimethylamine N-oxide and kynurenine in adults: a population-based study.Frontiers in nutrition · 2024Article
- From nitrate to NO: potential effects of nitrate-reducing bacteria on systemic health and disease.European journal of medical research · 2023Review
- Metatranscriptomic Analyses Reveal Important Roles of the Gut Microbiome in Primate Dietary Adaptation.Genes · 2023Article
- Long-term inorganic nitrate administration protects against ovariectomy-induced osteoporosis in rats.EXCLI journal · 2022Article
- Nitrate-induced improvements in exercise performance are coincident with exuberant changes in metabolic genes and the metabolome in zebrafish (Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- The role of gut microbiota in bone homeostasis.Bone & joint research · 2021Article
- Role of Oral and Gut Microbiota in Dietary Nitrate Metabolism and Its Impact on Sports Performance.Nutrients · 2020Review
- Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity.Journal of clinical biochemistry and nutrition · 2020Article
- Nitrate and nitrite exposure leads to mild anxiogenic-like behavior and alters brain metabolomic profile in zebrafish.PloS one · 2020Article
- Ovariectomized rat model of osteoporosis: a practical guide.EXCLI journal · 2020Review
- Treatment with Nitrate, but Not Nitrite, Lowers the Oxygen Cost of Exercise and Decreases Glycolytic Intermediates While Increasing Fatty Acid Metabolites in Exercised Zebrafish.The Journal of nutrition · 2019Article
- Increasing dietary nitrate has no effect on cancellous bone loss or fecal microbiome in ovariectomized rats.Molecular nutrition & food research · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
scopeStudies suggest diets rich in fruit and vegetables reduce bone loss, although the specific compounds responsible are unknown. Substrates for endogenous nitric oxide (NO) production, including organic nitrates and dietary nitrate, may support NO production in age-related conditions, including osteoporosis. We investigated the capability of dietary nitrate to improve NO bioavailability, reduce bone turnover and loss. METHODS AND
resultsSix-month-old Sprague Dawley rats [30 ovariectomized (OVX) and 10 sham-operated (sham)] were randomized into three groups: (i) vehicle (water) control, (ii) low-dose nitrate (LDN, 0.1 mmol nitrate/kg bw/day), or (iii) high-dose nitrate (HDN, 1.0 mmol nitrate/kg bw/day) for three weeks. The sham received vehicle. Serum bone turnover markers; bone mass, mineral density, and quality; histomorphometric parameters; and fecal microbiome were examined. Three weeks of LDN or HDN improved NO bioavailability in a dose-dependent manner. OVX resulted in cancellous bone loss, increased bone turnover, and fecal microbiome changes. OVX increased relative abundances of Firmicutes and decreased Bacteroideceae and Alcaligenaceae. Nitrate did not affect the skeleton or fecal microbiome.
conclusionThese data indicate that OVX affects the fecal microbiome and that the gut microbiome is associated with bone mass. Three weeks of nitrate supplementation does not slow bone loss or alter the fecal microbiome in OVX.
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