ArticleGenes & development2016
The piggyBac transposon-derived genes TPB1 and TPB6 mediate essential transposon-like excision during the developmental rearrangement of key genes in Tetrahymena thermophila.
Article in Genes & development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Relaxed DNA substrate specificity of transposases involved in programmed genome rearrangement.Nucleic acids research · 2025Article
- Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.Microbiology and molecular biology reviews : MMBR · 2023Review
- Structure and dynamics of the contractile vacuole complex in Tetrahymena thermophila.Journal of cell science · 2023Article
- VPS8D, a CORVET subunit, is required to maintain the contractile vacuole complex inbioRxiv : the preprint server for biology · 2023Article
- Origins of genome-editing excisases as illuminated by the somatic genome of the ciliateProceedings of the National Academy of Sciences of the United States of America · 2023Article
- MITE infestation accommodated by genome editing in the germline genome of the ciliateProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Structural insights into the evolution of the RAG recombinase.Nature reviews. Immunology · 2022Review
- Small RNA-directed DNA elimination: the molecular mechanism and its potential for genome editing.RNA biology · 2021Review
- Massive colonization of protein-coding exons by selfish genetic elements in Paramecium germline genomes.PLoS biology · 2021Article
- The unusual structure of the PiggyMac cysteine-rich domain reveals zinc finger diversity in PiggyBac-related transposases.Mobile DNA · 2021Article
- Programmed genome rearrangements in ciliates.Cellular and molecular life sciences : CMLS · 2020Review
- Comprehensive Chromosome End Remodeling during Programmed DNA Elimination.Current biology : CB · 2020Article
- Roles of Noncoding RNAs in Ciliate Genome Architecture.Journal of molecular biology · 2020Review
- Functional diversification of Paramecium Ku80 paralogs safeguards genome integrity during precise programmed DNA elimination.PLoS genetics · 2020Article
- Identification of RAG-like transposons in protostomes suggests their ancient bilaterian origin.Mobile DNA · 2020Article
- Host-transposon interactions: conflict, cooperation, and cooption.Genes & development · 2019Review
- Boundaries of eliminated heterochromatin of Tetrahymena are positioned by the DNA-binding protein Ltl1.Nucleic acids research · 2019Article
- Diversification of small RNA amplification mechanisms for targeting transposon-related sequences in ciliates.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Evolutionary origins and impacts of genome architecture in ciliates.Annals of the New York Academy of Sciences · 2019Review
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Ciliated protozoans perform extreme forms of programmed somatic DNA rearrangement during development. The model ciliate Tetrahymena thermophila removes 34% of its germline micronuclear genome from somatic macronuclei by excising thousands of internal eliminated sequences (IESs), a process that shares features with transposon excision. Indeed, piggyBac transposon-derived genes are necessary for genome-wide IES excision in both Tetrahymena (TPB2 [Tetrahymena piggyBac-like 2] and LIA5) and Paramecium tetraurelia (PiggyMac). T. thermophila has at least three other piggyBac-derived genes: TPB1, TPB6, and TPB7 Here, we show that TPB1 and TPB6 excise a small, distinct set of 12 unusual IESs that disrupt exons. TPB1-deficient cells complete mating, but their progeny exhibit slow growth, giant vacuoles, and osmotic shock sensitivity due to retention of an IES in the vacuolar gene DOP1 (Dopey domain-containing protein). Unlike most IESs, TPB1-dependent IESs have piggyBac-like terminal inverted motifs that are necessary for excision. Transposon-like excision mediated by TPB1 and TPB6 provides direct evidence for a transposon origin of not only IES excision machinery but also IESs themselves. Our study highlights a division of labor among ciliate piggyBac-derived genes, which carry out mutually exclusive categories of excision events mediated by either transposon-like features or RNA-directed heterochromatin.
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