ArticleProceedings of the National Academy of Sciences of the United States of America2017
Imaging proteins at the single-molecule level.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Triple-Helix Biomolecules Construct Self-Adaptive Multi-Phase Interfaces for Dendrites Immunized Zinc Metal Anodes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Clean Electrospray Deposition of Porphyrin Molecules and Polyethylene Glycol Chains onto a Au(100) Surface.The journal of physical chemistry. C, Nanomaterials and interfaces · 2026Article
- How to image single isolated atoms by using coherent low-energy electrons.Methods in microscopy · 2025Article
- Electrospray-based sample preparation for high-resolution protein structure elucidation by cryo-EM.Clinical and translational medicine · 2025Article
- Optical control of single-atom dynamics in plasmonic nanogaps.Science advances · 2025Article
- Scanning Probe Microscopy Characterization of Biomolecules enabled by Mass-Selective, Soft-landing Electrospray Ion Beam Deposition.Chemphyschem : a European journal of chemical physics and physical chemistry · 2024Article
- Ultra-Nonlinear Subcycle Photoemission of Few-Electron States from Sharp Gold Nanotapers.Nano letters · 2024Article
- Double-slit holography-a single-shot lensless imaging technique.Scientific reports · 2024Article
- The Complementarity of Nuclear Magnetic Resonance and Native Mass Spectrometry in Probing Protein-Protein Interactions.Advances in experimental medicine and biology · 2024Review
- Imaging conformations of holo- and apo-transferrin on the single-molecule level by low-energy electron holography.Scientific reports · 2023Article
- Electrospray ion beam deposition plus low-energy electron holography as a tool for imaging individual biomolecules.Essays in biochemistry · 2023Article
- Landing Proteins on Graphene Trampoline Preserves Their Gas-Phase Folding on the Surface.ACS central science · 2023Article
- Review
- Matrix-Landing Mass Spectrometry for Electron Microscopy Imaging of Native Protein Complexes.Analytical chemistry · 2022Article
- Article
- Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition.Faraday discussions · 2022Article
- Article
- Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition.PNAS nexus · 2022Article
- Scanning Tunneling Microscopy of Biological Structures: An Elusive Goal for Many Years.Nanomaterials (Basel, Switzerland) · 2022Review
- Three-dimensional structure determination of protein complexes using matrix-landing mass spectrometry.Nature communications · 2022Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process.
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