ArticleFEMS yeast research2017
Elimination of sucrose transport and hydrolysis in Saccharomyces cerevisiae: a platform strain for engineering sucrose metabolism.
Article in FEMS yeast research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
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- Transcriptome analysis and reverse engineering verification of SNZ3Biotechnology for biofuels and bioproducts · 2025Article
- Optogenetic spatial patterning of cooperation in yeast populations.Nature communications · 2024Article
- Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain.Journal of fungi (Basel, Switzerland) · 2023Article
- Novel Evolutionary Engineering Approach to Alter Substrate Specificity of Disaccharide Transporter Mal11 inJournal of fungi (Basel, Switzerland) · 2022Article
- Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- Evaluation of the Fermentative Capacity ofIndian journal of microbiology · 2020Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- HimalayanApplied and environmental microbiology · 2019Article
- In vivo recombination of Saccharomyces eubayanus maltose-transporter genes yields a chimeric transporter that enables maltotriose fermentation.PLoS genetics · 2019Article
- Pervasive function and evidence for selection across standing genetic variation in S. cerevisiae.Nature communications · 2019Article
- Allele-specific genome editing using CRISPR-Cas9 is associated with loss of heterozygosity in diploid yeast.Nucleic acids research · 2019Article
- A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains.FEMS yeast research · 2019Article
- Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes inFrontiers in microbiology · 2018Article
- Evolutionary Engineering in Chemostat Cultures for Improved Maltotriose Fermentation Kinetics inFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many relevant options to improve efficacy and kinetics of sucrose metabolism in Saccharomyces cerevisiae and, thereby, the economics of sucrose-based processes remain to be investigated. An essential first step is to identify all native sucrose-hydrolysing enzymes and sucrose transporters in this yeast, including those that can be activated by suppressor mutations in sucrose-negative strains. A strain in which all known sucrose-transporter genes (MAL11, MAL21, MAL31, MPH2, MPH3) were deleted did not grow on sucrose after 2 months of incubation. In contrast, a strain with deletions in genes encoding sucrose-hydrolysing enzymes (SUC2, MAL12, MAL22, MAL32) still grew on sucrose. Its specific growth rate increased from 0.08 to 0.25 h-1 after sequential batch cultivation. This increase was accompanied by a 3-fold increase of in vitro sucrose-hydrolysis and isomaltase activities, as well as by a 3- to 5-fold upregulation of the isomaltase-encoding genes IMA1 and IMA5. One-step Cas9-mediated deletion of all isomaltase-encoding genes (IMA1-5) completely abolished sucrose hydrolysis. Even after 2 months of incubation, the resulting strain did not grow on sucrose. This sucrose-negative strain can be used as a platform to test metabolic engineering strategies and for fundamental studies into sucrose hydrolysis or transport.
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