ArticleJournal of ovarian research2017
Quantifying growing versus non-growing ovarian follicles in the mouse.
Article in Journal of ovarian research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- TSG FromFood science & nutrition · 2026Article
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- Ovarian influence on circadian and infradian modulation of nicotine-seeking in female mice.Journal of neuroendocrinology · 2026Article
- Protective Effect of Glutathione Supplementation On Mouse Ovarian Tissue Vitrification and Autologous Transplantation.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Three-dimensional visualisation of human ovarian follicles using a whole-mount immunolabelling and optical tissue clearing method.Reproduction & fertility · 2025Article
- Evaluation of gibberellic acid toxicity on pre and postnatal ovarian development and potential protective effect of selenium in albino rats: histological, immunohistochemical, and biochemical study.Anatomy & cell biology · 2025Article
- Ovarian-Specific Cyp17A1 Overexpression in Female Mice: A Novel Model of Endogenous Testosterone Excess.Endocrinology · 2025Article
- Depletion ofInternational journal of molecular sciences · 2025Article
- Evaluation of Synthetic GnRH-Analog Peforelin with Regard to Oocyte Differentiation and Follicular Development in C57BL/6J Mice.Animals : an open access journal from MDPI · 2024Article
- EPAS1 expression contributes to maintenance of the primordial follicle pool in the mouse ovary.Scientific reports · 2024Article
- Early postnatal alterations in follicular stress response and survival in a mouse model of Classic Galactosemia.Journal of ovarian research · 2022Article
- Harnessing the Power of Purple Sweet Potato Color andInternational journal of molecular sciences · 2022Article
- Selective loss of kisspeptin signaling in oocytes causes progressive premature ovulatory failure.Human reproduction (Oxford, England) · 2022Article
- Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (Animals : an open access journal from MDPI · 2021Article
- Ectopic expression of CGG-repeats alters ovarian response to gonadotropins and leads to infertility in a murine FMR1 premutation model.Human molecular genetics · 2021Article
- Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi-Cox staining approach.Scientific reports · 2021Article
- Epigenetic effects of Bisphenol A on granulosa cells of mouse follicles during in vitro culture: An experimental study.International journal of reproductive biomedicine · 2021Article
- Control of Murine Primordial Follicle Growth Activation by IκB/NFκB Signaling.Reproductive sciences (Thousand Oaks, Calif.) · 2020Article
- The molecular mechanisms that underlie fragile X-associated premature ovarian insufficiency: is it RNA or protein based?Molecular human reproduction · 2020Review
- Effect of neuronal nitric oxide synthase serine-1412 phosphorylation on hypothalamic-pituitary-ovarian function and leptin response.Biology of reproduction · 2020Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA standard histomorphometric approach has been used for nearly 40 years that identifies atretic (e.g., dying) follicles by counting the number of pyknotic granulosa cells (GC) in the largest follicle cross-section. This method holds that if one pyknotic granulosa nucleus is seen in the largest cross section of a primary follicle, or three pyknotic cells are found in a larger follicle, it should be categorized as atretic. Many studies have used these criteria to estimate the fraction of atretic follicles that result from genetic manipulation or environmental insult. During an analysis of follicle development in a mouse model of Fragile X premutation, we asked whether these 'historical' criteria could correctly identify follicles that were not growing (and could thus confirmed to be dying).
methodsReasoning that the fraction of mitotic GC reveals whether the GC population was increasing at the time of sample fixation, we compared the number of pyknotic nuclei to the number of mitotic figures in follicles within a set of age-matched ovaries.
resultsWe found that, by itself, pyknotic nuclei quantification resulted in high numbers of false positives (improperly categorized as atretic) and false negatives (improperly categorized intact). For preantral follicles, scoring mitotic and pyknotic GC nuclei allowed rapid, accurate identification of non-growing follicles with 98% accuracy. This method most often required the evaluation of one follicle section, and at most two serial follicle sections to correctly categorize follicle status. For antral follicles, we show that a rapid evaluation of follicle shape reveals which are intact and likely to survive to ovulation.
conclusionsCombined, these improved, non-arbitrary methods will greatly improve our ability to estimate the fractions of growing/intact and non-growing/atretic follicles in mouse ovaries.
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