Evidence map›Paper›PMID 28086873›Full record

ArticleBMC genomics2017

Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer.

Guixian Wu, Yukai Qi, Xiaoyi Liu, Ning Yang, Guiyun Xu, Liying Liu, Xianyao Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 39 citations in OpenAlex.

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  17. Chicken gga-miR-1306-5p targetsJournal of animal science and biotechnology · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Guixian WuCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Yukai QiCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Xiaoyi LiuCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Ning YangCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Guiyun XuCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Liying LiuCollege of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China. lyliu@sdau.edu.cn.
Xianyao LiCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271018, China. xyli@sdau.edu.cn.ORCID 0000-0002-9904-4809
Shandong Agricultural University · CNChina Agricultural University · CNShandong Center for Disease Control and Prevention · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSalmonella enterica serovar Enteritidis (SE) is a food-borne pathogen and of great threat to human health through consuming the contaminated poultry products. MicroRNAs (miRNAs) play an important role in different biological activities and have been shown to regulate the innate immunity in the bacterial infection. The objective of this study is to identify miRNAs associated with SE infection in laying chicken cecum.

resultsAverage number of reads of three libraries constructed from infected and non-infected chickens was 12,476,156 and 10,866,976, respectively. There were 598 miRNAs including 194 potential novel miRNAs identified in which 37 miRNAs were significantly differentially expressed between infected and non-infected chickens. In total, 2897 unique target genes regulated by differentially expressed miRNAs were predicted, in which, 841 genes were uniquely regulated by up-regulated miRNAs (G1), 636 genes were uniquely regulated by down-regulated miRNAs (G2), and 1420 were co-regulated by both up and down- regulated miRNAs (G3). There were 118, 73 and 178 GO (Gene ontology) BP (Biological process) terms significantly enriched in G1, G2 and G3 groups, respectively. More immune-related GO BP terms than metabolism-related terms were found in G1. Expression of 12 immune-related genes of four differentially expressed miRNAs was detected through qRT-PCR. The regulatory direction of gga-miR-1416-5p, gga-miR-1662, and gga-miR-34a-5p were opposite with the target genes of TLR21, BCL10, TLR1LA, NOTCH2 and THBS1, respectively.

conclusionThe miRNAs contribute to the response to SE infection at the onset of egg laying through regulating the homeostasis between metabolism and immunity. The gga-miR-125b-5p, gga-miR-34a-5p, gga-miR-1416-5p and gga-miR-1662 could play an important role in SE infection through regulating their target genes. The finding herein will pave the foundation for the studies of microRNA regulation in SE infection in laying hens.

Indexed as

Salmonella enteritidisTranscriptomeAnimalsChickensCluster AnalysisGene Expression ProfilingGene Expression RegulationGene OntologyGenomeGenomicsHumansMetabolic Networks and PathwaysMicroRNAsProtein Interaction MappingProtein Interaction MapsReproducibility of ResultsMicroRNAsRNA, MessengerLaying chickenmiRNANext generation sequencingSalmonella enterica serovar Enteritidis

Identifiers

PMID28086873
PMCPMC5237128
OpenAlexW2576093325

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.