ArticleParasites & vectors2017
RNAi reveals proteins for metabolism and protein processing associated with Langat virus infection in Ixodes scapularis (black-legged tick) ISE6 cells.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Unravelling the biochemical aspects of the interaction between ticks and Leishmania using a tick cell line.Medical and veterinary entomology · 2026Article
- Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells.PLoS pathogens · 2025Article
- Pharmacological Agent GW4869 Inhibits Tick-Borne Langat Virus Replication to Affect Extracellular Vesicles Secretion.Viruses · 2025Article
- Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors.Nature communications · 2025Article
- The Impact of RNA Interference in Tick Research.Pathogens (Basel, Switzerland) · 2022Article
- Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost.Parasites & vectors · 2022Article
- Ambivalent Roles of Oxidative Stress in Triangular Relationships among Arthropod Vectors, Pathogens and Hosts.Antioxidants (Basel, Switzerland) · 2022Review
- Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections.International journal of molecular sciences · 2022Article
- Cationic Glycopolyelectrolytes for RNA Interference in Tick Cells.Biomacromolecules · 2022Article
- Alterations in arthropod and neuronal exosomes reduce virus transmission and replication in recipient cells.Extracellular vesicles and circulating nucleic acids · 2022Article
- An Experimental Murine Model to Study Acquisition Dynamics of Tick-Borne Langat Virus inFrontiers in microbiology · 2022Article
- Ixodid Tick Dissection and Tick Ex Vivo Organ Cultures for Tick-Borne Virus Research.Current protocols in microbiology · 2020Article
- Article
- The Tick Cell Biobank: A global resource for in vitro research on ticks, other arthropods and the pathogens they transmit.Ticks and tick-borne diseases · 2018Review
- The Use of Ex Vivo Organ Cultures in Tick-Borne Virus Research.ACS infectious diseases · 2018Review
- Mapping Arbovirus-Vector Interactions Using Systems Biology Techniques.Frontiers in cellular and infection microbiology · 2018Review
- Article
- Flavivirus Infection ofmBio · 2017Article
- Tick-Borne Viruses and Biological Processes at the Tick-Host-Virus Interface.Frontiers in cellular and infection microbiology · 2017Review
- A Roadmap for Tick-Borne Flavivirus Research in the "Omics" Era.Frontiers in cellular and infection microbiology · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundTick-borne flaviviruses (TBFs) cause thousands of human cases of encephalitis worldwide each year, with some TBF infections progressing to hemorrhagic fever. TBFs are of medical and veterinary importance and strategies to reduce flavivirus transmission by the tick vector may have significant application. Analyses of the proteome of ISE6 cells derived from the black legged tick, Ixodes scapularis infected with the TBF, Langat virus (LGTV), have provided insights into proteins and cellular processes involved with LGTV infection.
methodsRNA interference (RNAi)-induced knockdown of transcripts was used to investigate the role of ten tick proteins in the LGTV infection cycle in ISE6 cells. LGTV-infected cells were separately transfected with dsRNA corresponding to each gene of interest and the effect on LGTV genome replication and release of infectious virus was assessed by RT-qPCR and plaque assays, respectively.
resultsRNAi-induced knockdown of transcripts for two enzymes that likely function in amino acid, carbohydrate, lipid, terpenoid/polykeytide and vitamin metabolism, and a transcript for one protein of unknown function were associated with decreased replication of the LGTV genome and release of infectious virus from cells. The knockdown of transcripts for five enzymes predicted to function in metabolism, a protein likely associated with folding, sorting and degradation, and a protein of unknown function was associated with a decrease only in the amount of infectious LGTV released from cells.
conclusionsThese data suggest tick proteins potentially associated with metabolism and protein processing may be involved in LGTV infection of ISE6 cells. Our study provides information to begin to elucidate the function of these proteins and identify targets for the development of new interventions aimed at controlling the transmission of TBFs.
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