ArticleBMC infectious diseases2017
Genotypic characterization of Orientia tsutsugamushi from patients in two geographical locations in Sri Lanka.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Prediction of monthly occurrence number of scrub typhus in Ganzhou City, China, based on SARIMA and BPNN models.Infectious Disease Modelling · 2025Article
- The 100 top-cited articles on scrub typhus: a bibliometric analysis.Osong public health and research perspectives · 2021Article
- Performance of molecular and serologic tests for the diagnosis of scrub typhus.PLoS neglected tropical diseases · 2020Article
- Article
- Diagnosis of scrub typhus: recent advancements and challenges.3 Biotech · 2020Review
- A Bibliometric Analysis of Global Scientific Research on Scrub Typhus.BioMed research international · 2020Article
- Distribution of Orientia tsutsugamushi in rodents and mites collected from Central India.Environmental monitoring and assessment · 2019Article
- Outer Membrane Protein A Conservation amongTropical medicine and infectious disease · 2018Article
- An Update on Host-Pathogen Interplay and Modulation of Immune Responses during Orientia tsutsugamushi Infection.Clinical microbiology reviews · 2018Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo date more than 20 antigenically distinct strains of Orientia tsutsugamushi (OT) reported within the tsutsugamushi triangle that cause an undifferentiated acute febrile illness in humans. Genotypic characterization of OT in different geographic regions or within the same country, is important in order to establish effective diagnostics, clinical management and to develop effective vaccines. Genetic and antigenic characterization of OT causing human disease in OT-endemic regions is not known for Sri Lanka.
methodsAdult patients and children who were admitted with an acute febrile illness and presumed to having acute scrub typhus based on presence of an eschar and other supporting clinical features were recruited. Eschar biopsies and buffy coat samples collected from patients who were confirmed having OT by IFA were further studied by real time PCR (Orientia 47 kD) and nested PCR (Orientia 56 kD) amplification. DNA sequences were obtained for 56 kD gene amplicons and phylogenetic comparisons were analyzed using currently available data in GenBank [Neucleotide substitution per 100 residues, 1000 Bootstrap Trials].
resultsTwenty eschar biopsies (Location1,19, Location 2,1) and eight buffy coat samples (Location1,6, Location2,2) examined by real time PCR revealed Orientia amplicons in 16 samples. DNA sequences were obtained for the 56 kD gene amplicons in 12 eschars and 4 buffy coat samples. The genotypes of the Location1 samples revealed that, 7 exhibiting close homology with JP1 [distantly related to UT177 Thai (Karp related)], five had close homology with Kato strain, two had close homology with JGv and JG AF [Distantly related to Kawasaki M63383] and one had close homology with Gilliam strain. The Location 2 strain was closely related to Kuroki-Boryong L04956, the genotype which is distributed in far eastern Asia. Similar to other patients in the cohort this patient also had never travelled out of Sri Lanka.
conclusionsWe observed all three main OT genotypes in Sri Lanka, and the majority fell into Thai Karp related clade. These results demonstrate great antigenic diversity of OT in the studied areas of Sri Lanka.
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