Evidence map›Paper›PMID 28086071›Full record

ArticleThe Journal of general virology2017

155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication.

Ahmed O Hassan, Sai V Vemula, Anurag Sharma, Dinesh S Bangari, Krishna K Mishra, Suresh K Mittal

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ahmed O HassanDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Sai V VemulaDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Anurag SharmaDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Dinesh S BangariDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Krishna K MishraDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Suresh K MittalDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Purdue University West Lafayette · USCenter for Cancer Research · US

Funding

Adenoviral Vector-based Pandemic Influenza VaccineR01AI059374 · NIAID · PURDUE UNIVERSITY WEST LAFAYETTE · PI SURESH K MITTAL · 2005 to 2026
$5.0M
Nonhuman adenoviral vectors for gene therapyR01CA110176 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI MITTAL, SURESH K · 2005 to 2008
$915k
NCI NIH HHS R01 CA110176NIAID NIH HHS R01 AI059374
6 · The paper itself

Abstract

Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions.

Indexed as

Virus ReplicationAdenoviridaeAnimalsBlotting, NorthernBlotting, WesternCattleDNA Mutational AnalysisGene Expression ProfilingReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNAViral Structural ProteinsViral Structural Proteins

Identifiers

PMID28086071
PMCPMC5721929
OpenAlexW2588071895

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.