ArticleThe Journal of cell biology1989
The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 132 citations in OpenAlex.
- Article
- Evaluation of musculoskeletal phenotype of the G608G progeria mouse model with lonafarnib, pravastatin, and zoledronic acid as treatment groups.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Lamin B2 Modulates Nucleolar Morphology, Dynamics, and Function.Molecular and cellular biology · 2017Article
- An absence of nuclear lamins in keratinocytes leads to ichthyosis, defective epidermal barrier function, and intrusion of nuclear membranes and endoplasmic reticulum into the nuclear chromatin.Molecular and cellular biology · 2014Article
- Farnesylation of lamin B1 is important for retention of nuclear chromatin during neuronal migration.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Protein farnesylation and disease.Journal of inherited metabolic disease · 2012Review
- Epigenetic alterations in muscular disorders.Comparative and functional genomics · 2012Article
- Influence of lamin A on the mechanical properties of amphibian oocyte nuclei measured by atomic force microscopy.Biophysical journal · 2009Article
- Nuclear lamins and chromatin: when structure meets function.Advances in enzyme regulation · 2009Review
- Hutchinson-Gilford progeria mutant lamin A primarily targets human vascular cells as detected by an anti-Lamin A G608G antibody.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Dermal fibroblasts in Hutchinson-Gilford progeria syndrome with the lamin A G608G mutation have dysmorphic nuclei and are hypersensitive to heat stress.BMC cell biology · 2005Article
- A novel karyoskeletal protein: characterization of protein NO145, the major component of nucleolar cortical skeleton in Xenopus oocytes.Molecular biology of the cell · 2001Article
- Head and/or CaaX domain deletions of lamin proteins disrupt preformed lamin A and C but not lamin B structure in mammalian cells.Molecular biology of the cell · 2000Article
- Meiotic lamin C2: the unique amino-terminal hexapeptide GNAEGR is essential for nuclear envelope association.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Association of prenylated proteins with the plasma membrane and the inner nuclear membrane is mediated by the same membrane-targeting motifs.Molecular biology of the cell · 2000Article
- Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA.Molecular and cellular biology · 1998Article
- Functional dissection of YA, an essential, developmentally regulated nuclear lamina protein in Drosophila melanogaster.Molecular and cellular biology · 1998Article
- Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae.The Journal of cell biology · 1997Article
- Disruption of nuclear lamin organization alters the distribution of replication factors and inhibits DNA synthesis.The Journal of cell biology · 1997Article
- Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association.Molecular biology of the cell · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed the interaction of soluble nuclear lamins with the nuclear envelope by microinjection of normal and mutated lamins into the cytoplasm of Xenopus laevis oocytes. Our results demonstrate that the conserved cysteine of the carboxy-terminal tetrapeptide Cys Ala/Ser Ile Met of lamins is essential for their association with the nuclear envelope. Removal of this sequence or replacement of the cysteine by serine resulted in Xenopus lamin L1 remaining in a soluble, non-envelope-associated state within the nucleus. Similar mutations of Xenopus lamin A resulted in only partial reduction of nuclear envelope association, indicating that lamin A contains additional signals that can partially compensate for the lack of the cysteine. Mammalian lamin C lacks this tetrapeptide and is not associated with the nuclear envelope in our experimental system. Cloning of the tetrapeptide Cys Ala Ile Met to the carboxy terminus of human lamin C resulted in lamin being found in a nuclear envelope-associated form in oocytes. Mutations at the amino terminus and in the alpha-helical region of lamin L1 revealed that the carboxy terminus mediates the association of lamins with the nuclear envelope; however, this alone is insufficient for maintenance of a stable association with the nuclear envelope.
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