ArticleApplied microbiology and biotechnology2017
McrA primers for the detection and quantification of the anaerobic archaeal methanotroph 'Candidatus Methanoperedens nitroreducens'.
Article in Applied microbiology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 95 citations in OpenAlex.
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- A fixed methane filter maximizes freshwater emissions under warming.Nature climate change · 2026Article
- Methane-cycling microbial communities are spatially structured, seasonally dynamic, and functionally coupled in sediments of two nearby eutrophic hydroelectric reservoirs.Frontiers in microbiology · 2026Article
- Spatiotemporal variations and environmental drivers of denitrifying anaerobic methane oxidizers inFrontiers in microbiology · 2025Article
- Group-specific Quantification of mcrA genes of Methanogenic Archaea and "Candidatus Methanoperedens" by Digital PCR.Microbes and environments · 2025Article
- Vertical distribution of Candidatus Methylomirabilis and Methanoperedens in agricultural soils.Applied microbiology and biotechnology · 2024Article
- The Effects of Model Insoluble Copper Compounds in a Sedimentary Environment on Denitrifying Anaerobic Methane Oxidation (DAMO) Enrichment.Microorganisms · 2024Article
- Review
- Detection and Quantification of Candidatus Methanoperedens-Like Archaea in Freshwater Wetland Soils.Microbial ecology · 2023Article
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- Aerobic and anaerobic methane oxidation in a seasonally anoxic basin.Limnology and oceanography · 2022Article
- Unraveling Nitrogen, Sulfur, and Carbon Metabolic Pathways and Microbial Community Transcriptional Responses to Substrate Deprivation and Toxicity Stresses in a Bioreactor Mimicking Anoxic Brackish Coastal Sediment Conditions.Frontiers in microbiology · 2022Article
- Biogeographic distributions of microbial communities associated with anaerobic methane oxidation in the surface sediments of deep-sea cold seeps in the South China Sea.Frontiers in microbiology · 2022Article
- Article
- Linking microbial community and biological functions to redox potential during black-odor river sediment remediation.Environmental science and pollution research international · 2020Article
- Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant.Applied microbiology and biotechnology · 2020Article
- Anaerobic methanotrophic archaea of the ANME-2d clade feature lipid composition that differs from other ANME archaea.FEMS microbiology ecology · 2019Article
- Response of the Anaerobic Methanotroph "Applied and environmental microbiology · 2018Article
- Long-term nitrate removal through methane-dependent denitrification microorganisms in sequencing batch reactors fed with only nitrate and methane.AMB Express · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nitrogen and methane cycles are important biogeochemical processes. Recently, 'Candidatus Methanoperedens nitroreducens,' archaea that catalyze nitrate-dependent anaerobic oxidation of methane (AOM), were enriched, and their genomes were analyzed. Diagnostic molecular tools for the sensitive detection of 'Candidatus M. nitroreducens' are not yet available. Here, we report the design of two novel mcrA primer combinations that specifically target the alpha sub-unit of the methyl-coenzyme M reductase (mcrA) gene of 'Candidatus M. nitroreducens'. The first primer pair produces a fragment of 186-bp that can be used to quantify 'Candidatus M. nitroreducens' cells, whereas the second primer pair yields an 1191-bp amplicon that is with sufficient length and well suited for more detailed phylogenetic analyses. Six different environmental samples were evaluated with the new qPCR primer pair, and the abundances were compared with those determined using primers for the 16S rRNA gene. The qPCR results indicated that the number of copies of the 'Candidatus M. nitroreducens' mcrA gene was highest in rice field soil, with 5.6 ± 0.8 × 10
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