ArticlePlant biotechnology journal2017
Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- A toolkit for plant lipid engineering: Surveying the efficacies of lipogenic factors for accumulating specialty lipids.Frontiers in plant science · 2022Pooled it
- Lipidomic profiling of Arabidopsis chloroplast protein phosphatase SLP1 mutants reveals altered diurnal lipid remodeling.BBA advances · 2026Article
- Identification and Characterization of Lipid Droplet-Associated Protein (LDAP) Isoforms from Tung Tree (Plants (Basel, Switzerland) · 2025Article
- Inducible expression of DEFECTIVE IN ANTHER DEHISCENCE 1 enhances triacylglycerol accumulation and lipid droplet formation in vegetative tissues.The Plant journal : for cell and molecular biology · 2025Article
- The Plasma Membrane H+-ATPase Promoter Driving the Expression ofJournal of fungi (Basel, Switzerland) · 2024Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- Tung tree stearoyl-acyl carrier protein Δ9 desaturase improves oil content and cold resistance ofFrontiers in plant science · 2023Article
- Transcriptome analysis of five different tissues of bitter gourd (Momordica charantia L.) fruit identifies full-length genes involved in seed oil biosynthesis.Scientific reports · 2022Article
- Functional analysis of alternative castor bean DGAT enzymes.Genetics and molecular biology · 2022Article
- Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (Plants (Basel, Switzerland) · 2021Article
- Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis forFrontiers in plant science · 2021Article
- AKR2A participates in the regulation of cotton fibre development by modulating biosynthesis of very-long-chain fatty acids.Plant biotechnology journal · 2020Article
- Plant Biosystems Design for a Carbon-Neutral Bioeconomy.Biodesign research · 2020Review
- Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala.BMC plant biology · 2019Article
- Genome-wide identification and functional analysis of oleosin genes in Brassica napus L.BMC plant biology · 2019Article
- Mouse lipogenic proteins promote the co-accumulation of triacylglycerols and sesquiterpenes in plant cells.Planta · 2019Article
- Arabidopsis: the original plant chassis organism.Plant cell reports · 2018Review
- Plant Lipid Droplets and Their Associated Proteins: Potential for Rapid Advances.Plant physiology · 2018Review
- Response of high leaf-oil Arabidopsis thaliana plant lines to biotic or abiotic stress.Plant signaling & behavior · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops. Here, we determined whether the fatty acid composition of leaf oil could be engineered to accumulate unusual fatty acids. Eleostearic acid (ESA) is a conjugated fatty acid produced in seeds of the tung tree (Vernicia fordii) and has both industrial and nutritional end-uses. Arabidopsis thaliana lines with elevated leaf oil were first generated by transforming wild-type, cgi-58 or pxa1 mutants (the latter two of which contain mutations disrupting fatty acid breakdown) with the diacylglycerol acyltransferases (DGAT1 or DGAT2) and/or oleosin genes from tung. High-leaf-oil plant lines were then transformed with tung FADX, which encodes the fatty acid desaturase/conjugase responsible for ESA synthesis. Analysis of lipids in leaves revealed that ESA was efficiently excluded from phospholipids, and co-expression of tung FADX and DGAT2 promoted a synergistic increase in leaf oil content and ESA accumulation. Taken together, these results provide a new approach for increasing leaf oil content that is coupled with accumulation of unusual fatty acids. Implications for production of biofuels, bioproducts, and plant-pest interactions are discussed.
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