Evidence map›Paper›PMID 28083843›Full record

ArticleDigestive diseases and sciences2017

MicroRNA-19b Expression in Human Biliary Atresia Specimens and Its Role in BA-Related Fibrosis.

Dong Zhao, Yi Luo, Yun Xia, Jian-Jun Zhang, Qiang Xia

Abstract read
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In one paragraph

Article in Digestive diseases and sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. MicroRNAs in chronic pediatric diseases (Review).Experimental and therapeutic medicine · 2024
    Review
  2. Review
  3. Article
  4. Article
  5. Observational
  6. Review
  7. α-Smooth muscle actin expression predicts the outcome of Kasai portoenterostomy in biliary atresia.Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Dong ZhaoDepartment of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Rd., No. 1630, Shanghai, 200127, China.
Yi LuoDepartment of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Rd., No. 1630, Shanghai, 200127, China.
Yun XiaDepartment of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Rd., No. 1630, Shanghai, 200127, China.
Jian-Jun ZhangDepartment of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Rd., No. 1630, Shanghai, 200127, China.
Qiang XiaDepartment of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Rd., No. 1630, Shanghai, 200127, China. xiaqiang@shsmu.edu.cn.
Renji Hospital · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

aimBiliary atresia (BA) is a pediatric liver disease with unknown underlying etiology. MicroRNAs (miRNAs) represent a family of small noncoding RNAs. Among them, miR-19b has been suggested to function in the diseased liver. We therefore decided to investigate its potential role in BA.

methodsWe used infant-derived specimens to analyze miR-19b expression in a tissue- and cell-specific fashion, predicted interaction with genes, and finally performed a functional study in vitro.

resultsPatients with BA showed significantly lower miR-19b level in liver compared with controls, and pediatric end-stage liver disease (PELD) score was inversely correlated with miR-19b level. In vitro, miR-19b was significantly downregulated in activated hepatic stellate cells (HSCs) and exerted inhibitory effects on HSC activation, as confirmed by decreased alpha-smooth muscle actin (α-SMA) and type I collagen expression. Moreover, one mRNA target gene (TGFβR2) was identified. Computational prediction of miR-19b binding to the 3'-untranslated region (UTR) of TGFβR2 was validated by luciferase reporter assay. Furthermore, miR-19b mimic negatively regulated transforming growth factor-beta (TGF-β) signaling components, as demonstrated by decreased drosophila mothers against decapentaplegic homolog 3 (SMAD3) expression and blocking of TGF-β-induced expression of a1(I) and a2(I) procollagen miRNAs.

conclusionsOur data indicate that miR-19b may be involved in BA-related fibrosis.

Indexed as

Biliary AtresiaLiver Cirrhosis3' Untranslated RegionsActinsCell ProliferationCells, CulturedCollagen Type IDown-RegulationHepatic Stellate CellsHumansInfantLiverMicroRNAsProtein Serine-Threonine KinasesReceptors, Transforming Growth Factor betaReceptor, Transforming Growth Factor-beta Type II3' Untranslated RegionsACTA2 protein, humanActinsCollagen Type IMicroRNAsMIRN19A microRNA, humanProtein Serine-Threonine KinasesReceptors, Transforming Growth Factor betaReceptor, Transforming Growth Factor-beta Type IIBiliary atresiaFibrosisMicroRNA-19b

Identifiers

PMID28083843
OpenAlexW2572759951

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.