ArticleStructural dynamics (Melville, N.Y.)2017
Structural enzymology using X-ray free electron lasers.
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Article in Structural dynamics (Melville, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64citing papers in PubMed
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Who cites it
64 citing papers in PubMed.
- Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography.IUCrJ · 2026Article
- Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human NQO1.Communications chemistry · 2026Article
- Sensitive detection of structural dynamics using a statistical framework for comparative crystallography.Science advances · 2025Article
- Combining Reactive Quantum-Mechanical Molecular-Dynamics Simulations with Mutagenesis, Crystallography, and Enzyme Kinetics to Reveal Plausible Steps of Isocyanide Hydratase Catalysis.Journal of chemical information and modeling · 2025Article
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Article
- Protein crystallization and structure determination at room temperature in the CrystalChip.FEBS open bio · 2025Article
- Article
- Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.IUCrJ · 2025Article
- Innovative Strategies in X-ray Crystallography for Exploring Structural Dynamics and Reaction Mechanisms in Metabolic Disorders.Journal of personalized medicine · 2024Review
- A snapshot love story: what serial crystallography has done and will do for us.Acta crystallographica. Section D, Structural biology · 2024Review
- Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron.bioRxiv : the preprint server for biology · 2024Article
- Time-resolved crystallography of boric acid binding to the active site serine of the β-lactamase CTX-M-14 and subsequent 1,2-diol esterification.Communications chemistry · 2024Article
- TheJournal of applied crystallography · 2024Article
- Characterization of Biological Samples Using Ultra-Short and Ultra-Bright XFEL Pulses.Advances in experimental medicine and biology · 2024Article
- BioCARS: Synchrotron facility for probing structural dynamics of biological macromolecules.Structural dynamics (Melville, N.Y.) · 2024Article
- Heterogeneity in M. tuberculosis β-lactamase inhibition by Sulbactam.Nature communications · 2023Article
- Room-temperature structural studies of SARS-CoV-2 protein NendoU with an X-ray free-electron laser.Structure (London, England : 1993) · 2023Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
50 authors.
Christopher KupitzPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Jose L OlmosDepartment of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
Mark HollDepartment of Physics, Arizona State University , Tempe, Arizona 85287, USA.
Lee TremblayMarbles Inc. , 1900 Belvedere Pl, Westfield, Indiana 46074, USA.
Kanupriya Pande
Suraj PandeyPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Dominik Oberthür
Mark HunterLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory , 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Mengning LiangLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory , 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Andrew AquilaLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory , 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Jason TenboerPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
George CalveyDepartment of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
Andrea KatzDepartment of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
Yujie ChenDepartment of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
Max O WiedornCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Juraj KnoskaCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Alke MeentsUniversity of Hamburg , Luruper Chaussee 149, 22761 Hamburg, Germany.
Valerio Majriani
Tyler NorwoodPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Ishwor PoudyalPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Thomas GrantHauptman-Woodward Institute, State University of New York at Buffalo , 700 Ellicott Street, Buffalo, New York 14203, USA.
Mitchell D MillerDepartment of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
Weijun XuDepartment of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
Aleksandra TolstikovaCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Andrew MorganCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Markus MetzCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Jose M Martin-GarciaSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
James D ZookSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Shatabdi Roy-ChowdhurySchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Jesse CoeSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Nirupa NagaratnamSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Domingo MezaSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Raimund FrommeSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Shibom BasuSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Matthias FrankLawrence Livermore National Laboratory , Livermore, California 94550, USA.
Thomas WhiteCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Anton BartyCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Sasa BajtCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Oleksandr YefanovCenter for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Henry N Chapman
Nadia ZatsepinDepartment of Physics, Arizona State University , Tempe, Arizona 85287, USA.
Garrett NelsonDepartment of Physics, Arizona State University , Tempe, Arizona 85287, USA.
Uwe WeierstallDepartment of Physics, Arizona State University , Tempe, Arizona 85287, USA.
John SpenceDepartment of Physics, Arizona State University , Tempe, Arizona 85287, USA.
Peter SchwanderPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Lois PollackDepartment of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
Petra FrommeSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
Abbas OurmazdPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
George N PhillipsDepartment of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
Marius SchmidtPhysics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Funding
ZWITTERION SURFACTANT DDMAB FOR IMPROV STABIL OF PROTEINP41RR001209 · NCRR · STANFORD UNIVERSITY · PI GONZALEZ, ANA M · 1985 to 2011
$38.9MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MTechnology development for biological imaging with x-ray free electron lasersR01GM117342 · NIGMS · LAWRENCE LIVERMORE NATIONAL SECURITY, LLC · PI FRANK, MATTHIAS · 2016 to 2024
$4.8MFemtosecond nano-crystallography of membrane proteinsR01GM095583 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2023
$4.3MEnzyme Discovery for Natural Product BiosynthesisU01GM098248 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PHILLIPS, GEORGE NEAL · 2011 to 2015
$2.8MTracking conformational states in enzyme catalysts using correlated X-ray scatterR01GM097463 · NIGMS · STANFORD UNIVERSITY · PI DONIACH, SEBASTIAN · 2012 to 2015
$1.7MRigidity and flexibility of large bio-molecular assembliesR01GM109456 · NIGMS · SMITH COLLEGE · PI STREINU, ILEANA · 2013 to 2017
$1.4MNCRR NIH HHS P41 RR001209NIGMS NIH HHS P41 GM103393NIGMS NIH HHS R01 GM095583NIGMS NIH HHS R01 GM097463NIGMS NIH HHS R01 GM109456NIGMS NIH HHS U01 GM098248
Abstract
Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from microseconds to seconds, and (iii) convenient reaction initiation. It outruns radiation damage by using femtosecond X-ray pulses allowing damage and chemistry to be separated. Here, we demonstrate that MISC is feasible at an X-ray free electron laser by studying the reaction of
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