ArticleNucleic acids research2017
Differential hepatitis C virus RNA target site selection and host factor activities of naturally occurring miR-122 3΄ variants.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 26 citations in OpenAlex.
- Circulating isomiRs May Be Superior Biomarkers Compared to Their Corresponding miRNAs: A Pilot Biomarker Study of Using isomiR-Ome to Detect Coronary Calcium-Based Cardiovascular Risk in Patients with NAFLD.International journal of molecular sciences · 2024Article
- One locus, several functional RNAs-emerging roles of the mechanisms responsible for the sequence variability of microRNAs.Biologia futura · 2023Review
- CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling.Nucleic acids research · 2023Article
- Transcriptional Inhibition of MicroRNA miR-122 by Small Molecules Reduces Hepatitis C Virus Replication in Liver Cells.Journal of medicinal chemistry · 2022Article
- DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity.Nucleic acids research · 2022Article
- TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance.Nature communications · 2022Article
- Dysregulation of Human Somatic piRNA Expression in Parkinson's Disease Subtypes and Stages.International journal of molecular sciences · 2022Article
- Global profiling and annotation of templated isomiRs dynamics acrossRNA biology · 2022Article
- A structured RNA motif locks Argonaute2:miR-122 onto the 5' end of the HCV genome.Nature communications · 2021Article
- Molecular Mechanisms of Nutrient-Mediated Regulation of MicroRNAs in Pancreatic β-cells.Frontiers in endocrinology · 2021Review
- isomiRs-Hidden Soldiers in the miRNA Regulatory Army, and How to Find Them?Biomolecules · 2020Review
- Stimulator of interferon genes (STING) is an essential proviral host factor for human rhinovirus species A and C.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evolutionary selection of pestivirus variants with altered or no microRNA dependency.Nucleic acids research · 2020Article
- IsomiRs: Expanding the miRNA repression toolbox beyond the seed.Biochimica et biophysica acta. Gene regulatory mechanisms · 2020Review
- Review
- A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools.Journal of visualized experiments : JoVE · 2019Article
- 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.Molecular cell · 2019Article
- TNRC6 proteins modulate hepatitis C virus replication by spatially regulating the binding of miR-122/Ago2 complexes to viral RNA.Nucleic acids research · 2019Article
- Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.Nature microbiology · 2019Article
- Poly(A)-specific ribonuclease sculpts the 3' ends of microRNAs.RNA (New York, N.Y.) · 2019Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
In addition to suppressing cellular gene expression, certain miRNAs potently facilitate replication of specific positive-strand RNA viruses. miR-122, a pro-viral hepatitis C virus (HCV) host factor, binds and recruits Ago2 to tandem sites (S1 and S2) near the 5΄ end of the HCV genome, stabilizing it and promoting its synthesis. HCV target site selection follows canonical miRNA rules, but how non-templated 3΄ miR-122 modifications impact this unconventional miRNA action is unknown. High-throughput sequencing revealed that a 22 nt miRNA with 3΄G ('22-3΄G') comprised <63% of total miR-122 in human liver, whereas other variants (23-3΄A, 23-3΄U, 21-3΄U) represented 11-17%. All loaded equivalently into Ago2, and when tested individually functioned comparably in suppressing gene expression. In contrast, 23-3΄A and 23-3΄U were more active than 22-3΄G in stabilizing HCV RNA and promoting its replication, whereas 21-3΄U was almost completely inactive. This lack of 21-3΄U HCV host factor activity correlated with reduced recruitment of Ago2 to the HCV S1 site. Additional experiments demonstrated strong preference for guanosine at nt 22 of miR-122. Our findings reveal the importance of non-templated 3΄ miR-122 modifications to its HCV host factor activity, and identify unexpected differences in miRNA requirements for host gene suppression versus RNA virus replication.
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