ArticleNucleic acids research2017
An optimized, broadly applicable piggyBac transposon induction system.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Highly efficient and low-mosaicismiScience · 2026Article
- Efficient generation of single-copy transgenic mice using piggyBat transposase from the little brown bat Myotis lucifugus†.Biology of reproduction · 2026Article
- Article
- Inducible Calling Cards: Developing Mouse Reagents for Experimentally Controlled Transposon InsertionbioRxiv : the preprint server for biology · 2025Article
- Single-cell technology grows up: Leveraging high-resolution omics approaches to understand neurodevelopmental disorders.Current opinion in neurobiology · 2025Review
- Non-viral generation of transgenic non-human primates via the piggyBac transposon system.Nature communications · 2025Article
- Carbonic anhydrase 2-derived drug-responsive domain regulates membrane-bound cytokine expression and function in engineered T cells.Communications biology · 2025Article
- Embryonic Lethal Phenotyping to Identify Candidate Genes Related with Birth Defects.International journal of molecular sciences · 2024Article
- Prospects and limitations of cumate-inducible lentivirus as a tool for investigating VEGF-A-mediated pathology in diabetic retinopathy.Scientific reports · 2024Article
- Effect of genomic and cellular environments on gene expression noise.Genome biology · 2024Article
- Measuring transcription factor binding and gene expression using barcoded self-reporting transposon calling cards and transcriptomes.NAR genomics and bioinformatics · 2022Article
- Article
- Genomic environments scale the activities of diverse core promoters.Genome research · 2022Article
- Combined effects of cisplatin and photon or proton irradiation in cultured cells: radiosensitization, patterns of cell death and cell cycle distribution.Journal of radiation research · 2020Article
- Article
- A viral toolkit for recording transcription factor-DNA interactions in live mouse tissues.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Enhancer Trapping and Annotation in Zebrafish Mediated with Sleeping Beauty, piggyBac and Tol2 Transposons.Genes · 2018Article
- Transposase mapping identifies the genomic targets of BAP1 in uveal melanoma.BMC medical genomics · 2018Article
- Drug-Inducible Control of Lethality Genes: A Low Background Destabilizing Domain Architecture Applied to the Gal4-UAS System in Drosophila.ACS synthetic biology · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The piggyBac (PB) transposon has been used in a number of biological applications. The insertion of PB transposons into the genome can disrupt genes or regulatory regions, impacting cellular function, so for many experiments it is important that PB transposition is tightly controlled. Here, we systematically characterize three methods for the post-translational control of the PB transposon in four cell lines. We investigated fusions of the PB transposase with ERT2 and two degradation domains (FKBP-DD, DHFR-DD), in multiple orientations, and determined (i) the fold-induction achieved, (ii) the absolute transposition efficiency of the activated construct and (iii) the effects of two inducer molecules on cellular transcription and function. We found that the FKBP-DD confers the PB transposase with a higher transposition activity and better dynamic range than can be achieved with the other systems. In addition, we found that the FKBP-DD regulates transposon activity in a reversible and dose-dependent manner. Finally, we showed that Shld1, the chemical inducer of FKBP-DD, does not interfere with stem cell differentiation, whereas tamoxifen has significant effects. We believe the FKBP-based PB transposon induction will be useful for transposon-mediated genome engineering, insertional mutagenesis and the genome-wide mapping of transcription factor binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.