ArticleNucleic acids research2017
Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 38 citations in OpenAlex.
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- Chromatin compaction precedes apoptosis in developing neurons.Communications biology · 2022Article
- Plasmonic hot spots reveal local conformational transitions induced by DNA double-strand breaks.Scientific reports · 2022Article
- HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein.Nucleic acids research · 2022Article
- Recent Microscopy Advances and the Applications to Huntington's Disease Research.Journal of Huntington's disease · 2022Review
- Recent progress on single-molecule localization microscopy.Biophysics reports · 2021Article
- Tackling Tumour Cell Heterogeneity at the Super-Resolution Level in Human Colorectal Cancer Tissue.Cancers · 2021Article
- Studying nanoscale structural alterations in cancer cells to evaluate ovarian cancer drug treatment, using transmission electron microscopy imaging.Physical biology · 2020Article
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- Sample drift estimation method based on speckle patterns formed by backscattered laser light.Biomedical optics express · 2019Article
- A guide to visualizing the spatial epigenome with super-resolution microscopy.The FEBS journal · 2019Review
- Super-Resolution Microscopy of Chromatin.Genes · 2019Review
- Super-resolution microscopy demystified.Nature cell biology · 2019Review
- Application of fluorescence lifetime imaging microscopy of DNA binding dyes to assess radiation-induced chromatin compaction changes.International journal of molecular sciences · 2018Article
- Super-resolution binding activated localization microscopy through reversible change of DNA conformation.Nucleus (Austin, Tex.) · 2018Review
- Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates.Frontiers in microbiology · 2017Review
- Article
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Authors and funding
9 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advanced light microscopy is an important tool for nanostructure analysis of chromatin. In this report we present a general concept for Single Molecule localization Microscopy (SMLM) super-resolved imaging of DNA-binding dyes based on modifying the properties of DNA and the dye. By careful adjustment of the chemical environment leading to local, reversible DNA melting and hybridization control over the fluorescence signal of the DNA-binding dye molecules can be introduced. We postulate a transient binding as the basis for our variation of binding-activated localization microscopy (BALM). We demonstrate that several intercalating and minor-groove binding DNA dyes can be used to register (optically isolate) only a few DNA-binding dye signals at a time. To highlight this DNA structure fluctuation-assisted BALM (fBALM), we applied it to measure, for the first time, nanoscale differences in nuclear architecture in model ischemia with an anticipated structural resolution of approximately 50 nm. Our data suggest that this approach may open an avenue for the enhanced microscopic analysis of chromatin nano-architecture and hence the microscopic analysis of nuclear structure aberrations occurring in various pathological conditions. It may also become possible to analyse nuclear nanostructure differences in different cell types, stages of development or environmental stress conditions.
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