ArticleJournal of biomechanics2017
Cellular cholesterol regulates monocyte deformation.
Article in Journal of biomechanics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Hyperspectral unmixing for Raman spectroscopy via physics-constrained autoencoders.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry.Nature communications · 2021Article
- The Entry and Egress of Monocytes in Atherosclerosis: A Biochemical and Biomechanical Driven Process.Cardiovascular therapeutics · 2021Review
- Microfluidic Point-of-Care Testing: Commercial Landscape and Future Directions.Frontiers in bioengineering and biotechnology · 2020Review
- Red blood cell deformability is diminished in patients with Chronic Fatigue Syndrome.Clinical hemorheology and microcirculation · 2019Article
- Review
- Influence of membrane cholesterol on monocyte chemotaxis.Cellular immunology · 2018Article
- Core-shell magnetoelectric nanorobot - A remotely controlled probe for targeted cell manipulation.Scientific reports · 2018Article
- Cholesterol Regulates Monocyte Rolling through CD44 Distribution.Biophysical journal · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The role of cholesterol content on monocyte biomechanics remains understudied despite the well-established link between cholesterol and monocytes/macrophages in atherosclerosis, and the effect on other cell types. In this work, we have investigated the effect of cholesterol on monocyte deformability and the underlying molecular mechanisms. We altered the baseline cholesterol in human monocytic cell line THP-1, and investigated the changes in monocyte deformability using a custom microfluidic platform and atomic force microscopy. We observed that the cholesterol depletion lowered deformability while enrichment increased deformability compared to untreated cells. As a consequence of altered deformability, cholesterol depleted cells spread more on collagen-coated surfaces with elongated morphology, whereas cholesterol enriched cells had a more rounded morphology. We observed that the decreased deformability in cholesterol depleted cells, despite an increase in the fluidity of the membrane, is due to an increase in phosphorylation of Protein Kinase C (PKC), which translates to a higher degree of actin polymerization. Together, our results highlight the importance of biophysical regulation of monocyte response to cholesterol levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.