ArticleHistochemistry1989
Light and electron microscopic detection of (3 Gal beta 1,4 GlcNAc beta 1) sequences in asparagine-linked oligosaccharides with the Datura stramonium lectin.
Article in Histochemistry, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- New Mutants of Epsilon Toxin fromToxins · 2022Article
- Lectin cytochemical characterization of the N- and O-linked oligosaccharides in the human rectum.The Histochemical journal · 2000Article
- The complex-type oligosaccharide binding lectin Datura stramonium agglutinin detects type II A muscle fibres in the branchial biceps from man and cat.Journal of muscle research and cell motility · 1997Article
- Histochemical demonstration of different types of poly-N-acetyllactosamine structures in human thyroid neoplasms using lectins and endo-beta-galactosidase digestion.The Histochemical journal · 1995Article
- Subcellular distribution of terminal alpha-D- and beta-D-galactosyl residues in Ehrlich tumour cells studied by lectin-gold techniques.Glycoconjugate journal · 1995Article
- Subcellular characterization of glycoproteins in the principal cells of human gallbladder. A lectin cytochemical study.Histochemistry · 1994Article
- Histochemistry of carbohydrates as performed by physical development procedures.The Histochemical journal · 1993Review
- Cytochemical characterization of glycoproteins in the developing acrosome of rats. An ultrastructural study using lectin histochemistry, enzymes and chemical deglycosylation.Histochemistry · 1992Article
- Sequences within and adjacent to the transmembrane segment of alpha-2,6-sialyltransferase specify Golgi retention.The EMBO journal · 1991Article
- DS28-6, a temperature-sensitive mutant of Chinese hamster ovary cells, expresses key phenotypic changes associated with brefeldin A treatment.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Glycosylation in intestinal epithelium.International review of cytology · 1991Review
- Contrasting of Lowicryl K4M thin sections.Histochemistry · 1990Article
- Lectin--digoxigenin conjugates: a new hapten system for glycoconjugate cytochemistry.Histochemistry · 1990Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Datura stramonium lectin recognizes with high affinity the disaccharide N-acetyllactosamine (Gal beta 1,4 GlcNAc). We have developed a highly specific cytochemical affinity technique in which an ovomucoid-gold complex serves as second step reagent for the visualization of this lectin bound to reactive sequences present in tissue sections. The lectin binding sites were detected in semithin and ultrathin sections of aldehyde-fixed and low temperature Lowicryl K4M embedded tissues. For light microscopical labeling the photochemical silver reaction for signal amplification was required. The application of this technique for the detection of N-acetyllactosamine containing asparagine-linked oligosaccharides in various intracellular organelles and the plasma membrane is demonstrated.
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