ArticleNucleic acids research2017
Mechanistic insights into type I toxin antitoxin systems in Helicobacter pylori: the importance of mRNA folding in controlling toxin expression.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
- The role of toxin-antitoxin systems inJournal of bacteriology · 2026Review
- An RNA structural switch controlling bacterial toxin translation.Nucleic acids research · 2026Article
- CoccoidVirulence · 2025Review
- Design of orthogonal and portable RNA devices for post-transcriptional regulation by reverse engineering type I toxin-antitoxin systems.Nucleic acids research · 2025Article
- Functional diversity and molecular interactions of small membrane proteins in bacteria.microLife · 2025Review
- Type I toxin-antitoxin systems in bacteria: from regulation to biological functions.EcoSal Plus · 2024Review
- An RNA pseudoknot mediates toxin translation and antitoxin inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Effects of C-Terminal Lys-Arg Residue of AapA1 Protein on Toxicity and Structural Mechanism.Toxins · 2023Article
- T1TAdb: the database of type I toxin-antitoxin systems.RNA (New York, N.Y.) · 2021Article
- Review
- Elevated Expression of Toxin TisB Protects Persister Cells against Ciprofloxacin but Enhances Susceptibility to Mitomycin C.Microorganisms · 2021Article
- Toxin-Antitoxin Systems - A New Player in Morphological Transformation of Antibiotic-ExposedFrontiers in cellular and infection microbiology · 2021Article
- RNA Regulated Toxin-Antitoxin Systems in Pathogenic Bacteria.Frontiers in cellular and infection microbiology · 2021Review
- Riboregulation in the Major Gastric PathogenFrontiers in microbiology · 2021Review
- Improved growth ofJournal of bacteriology · 2021Article
- A peptide of a type I toxin-antitoxin system inducesProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Small ToxicmBio · 2020Article
- Functional characterization of the type I toxin Lpt from Lactobacillus rhamnosus by fluorescence and atomic force microscopy.Scientific reports · 2019Article
- A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNA.eLife · 2019Article
- Maturation of atypical ribosomal RNA precursors in Helicobacter pylori.Nucleic acids research · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type I toxin-antitoxin (TA) systems have been identified in a wide range of bacterial genomes. Here, we report the characterization of a new type I TA system present on the chromosome of the major human gastric pathogen, Helicobacter pylori. We show that the aapA1 gene encodes a 30 amino acid peptide whose artificial expression in H. pylori induces cell death. The synthesis of this toxin is prevented by the transcription of an antitoxin RNA, named IsoA1, expressed on the opposite strand of the toxin gene. We further reveal additional layers of post-transcriptional regulation that control toxin expression: (i) transcription of the aapA1 gene generates a full-length transcript whose folding impedes translation (ii) a 3΄ end processing of this message generates a shorter transcript that, after a structural rearrangement, becomes translatable (iii) but this rearrangement also leads to the formation of two stem-loop structures allowing formation of an extended duplex with IsoA1 via kissing-loop interactions. This interaction ensures both the translation inhibition of the AapA1 active message and its rapid degradation by RNase III, thus preventing toxin synthesis under normal growth conditions. Finally, a search for homologous mRNA structures identifies similar TA systems in a large number of Helicobacter and Campylobacter genomes.
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